Systemic immunosuppression depletes peripheral blood regulatory B cells in patients with immune thrombocytopenia

Madeleine L Stimpson; Julia Wolf; Charbit Charbit; Emily L Williams; Philippa J P Lait; Lauren P Schewitz-Bowers; Richard W J Lee; Charlotte A Bradbury

doi:10.1111/bjh.19144

Systemic immunosuppression depletes peripheral blood regulatory B cells in patients with immune thrombocytopenia

Madeleine L Stimpson, Julia Wolf, Charbit Charbit, Emily L Williams, Philippa J P Lait, Lauren P Schewitz-Bowers, Richard W J Lee, Charlotte A Bradbury^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

3 Citations (Scopus)

Abstract

Regulatory B (Breg) cells are potentially implicated in the pathogenesis of Immune Thrombocytopenia (ITP). We analysed a prospective cohort of newly diagnosed steroid naïve ITP patients enrolled in the multicentre FLIGHT trial and found that the numbers of Bregs in their peripheral blood were similar to healthy controls. In contrast, Breg numbers were significantly reduced in ITP patients treated with systemic immunosuppression (glucocorticoids or mycophenolate mofetil). We also demonstrate that glucocorticoid treatment impairs Breg IL-10 production via an indirect T-cell mediated mechanism.

Original language	English
Pages (from-to)	644-648
Number of pages	5
Journal	British Journal of Haematology
Volume	204
Issue number	2
Early online date	12 Oct 2023
DOIs	https://doi.org/10.1111/bjh.19144
Publication status	Published - 6 Feb 2024

Bibliographical note

Funding Information:
This project is partly funded by the National Institute for Health Research (NIHR) under its Research for Patient Benefit (RfPB) Programme (Grant Reference Number PB‐PG‐0815‐20016 – CAB) and Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology (ELW, PJPL, LPS‐B and RWJL). The views expressed are those of the author(s) and not necessarily those of the NIHR or the Department of Health and Social Care. In addition, project‐specific funding was received through a grant from the British Medical Association.

Publisher Copyright:
© 2023 The Authors. British Journal of Haematology published by British Society for Haematology and John Wiley & Sons Ltd.

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title = "Systemic immunosuppression depletes peripheral blood regulatory B cells in patients with immune thrombocytopenia",

abstract = "Regulatory B (Breg) cells are potentially implicated in the pathogenesis of Immune Thrombocytopenia (ITP). We analysed a prospective cohort of newly diagnosed steroid na{\"i}ve ITP patients enrolled in the multicentre FLIGHT trial and found that the numbers of Bregs in their peripheral blood were similar to healthy controls. In contrast, Breg numbers were significantly reduced in ITP patients treated with systemic immunosuppression (glucocorticoids or mycophenolate mofetil). We also demonstrate that glucocorticoid treatment impairs Breg IL-10 production via an indirect T-cell mediated mechanism.",

author = "Stimpson, {Madeleine L} and Julia Wolf and Charbit Charbit and Williams, {Emily L} and Lait, {Philippa J P} and Schewitz-Bowers, {Lauren P} and Lee, {Richard W J} and Bradbury, {Charlotte A}",

note = "Funding Information: This project is partly funded by the National Institute for Health Research (NIHR) under its Research for Patient Benefit (RfPB) Programme (Grant Reference Number PB‐PG‐0815‐20016 – CAB) and Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology (ELW, PJPL, LPS‐B and RWJL). The views expressed are those of the author(s) and not necessarily those of the NIHR or the Department of Health and Social Care. In addition, project‐specific funding was received through a grant from the British Medical Association. Publisher Copyright: {\textcopyright} 2023 The Authors. British Journal of Haematology published by British Society for Haematology and John Wiley & Sons Ltd.",

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T1 - Systemic immunosuppression depletes peripheral blood regulatory B cells in patients with immune thrombocytopenia

AU - Stimpson, Madeleine L

AU - Wolf, Julia

AU - Charbit, Charbit

AU - Williams, Emily L

AU - Lait, Philippa J P

AU - Schewitz-Bowers, Lauren P

AU - Lee, Richard W J

AU - Bradbury, Charlotte A

N1 - Funding Information: This project is partly funded by the National Institute for Health Research (NIHR) under its Research for Patient Benefit (RfPB) Programme (Grant Reference Number PB‐PG‐0815‐20016 – CAB) and Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology (ELW, PJPL, LPS‐B and RWJL). The views expressed are those of the author(s) and not necessarily those of the NIHR or the Department of Health and Social Care. In addition, project‐specific funding was received through a grant from the British Medical Association. Publisher Copyright: © 2023 The Authors. British Journal of Haematology published by British Society for Haematology and John Wiley & Sons Ltd.

PY - 2024/2/6

Y1 - 2024/2/6

N2 - Regulatory B (Breg) cells are potentially implicated in the pathogenesis of Immune Thrombocytopenia (ITP). We analysed a prospective cohort of newly diagnosed steroid naïve ITP patients enrolled in the multicentre FLIGHT trial and found that the numbers of Bregs in their peripheral blood were similar to healthy controls. In contrast, Breg numbers were significantly reduced in ITP patients treated with systemic immunosuppression (glucocorticoids or mycophenolate mofetil). We also demonstrate that glucocorticoid treatment impairs Breg IL-10 production via an indirect T-cell mediated mechanism.

AB - Regulatory B (Breg) cells are potentially implicated in the pathogenesis of Immune Thrombocytopenia (ITP). We analysed a prospective cohort of newly diagnosed steroid naïve ITP patients enrolled in the multicentre FLIGHT trial and found that the numbers of Bregs in their peripheral blood were similar to healthy controls. In contrast, Breg numbers were significantly reduced in ITP patients treated with systemic immunosuppression (glucocorticoids or mycophenolate mofetil). We also demonstrate that glucocorticoid treatment impairs Breg IL-10 production via an indirect T-cell mediated mechanism.

U2 - 10.1111/bjh.19144

DO - 10.1111/bjh.19144

M3 - Article (Academic Journal)

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JO - British Journal of Haematology

JF - British Journal of Haematology

IS - 2

ER -

Systemic immunosuppression depletes peripheral blood regulatory B cells in patients with immune thrombocytopenia

Abstract

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