Systems Imaging of the Immune Synapse

Christoph Wuelfing, Rachel Ambler, Xiangtao RUAN, Robert F. Murphy

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

5 Citations (Scopus)
243 Downloads (Pure)


Three‐dimensional live cell imaging of the interaction of T cells with antigen presenting cells (APC) visualises the subcellular distributions of signalling intermediates during T cell activation at thousands of resolved positions within a cell. These information-rich maps of local protein concentrations are a valuable resource in understanding T cell signalling. Here, we describe a protocol for the efficient acquisition of such imaging data and their computational processing to create four-dimensional maps of local concentrations. This protocol allows quantitative analysis of T cell signalling as it occurs inside live cells with resolution in time and space across thousands of cells.
Original languageEnglish
Title of host publicationThe Immune Synapse
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
ISBN (Electronic)978-1-4939-6881-7
ISBN (Print)978-1-4939-6879-4
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


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