Taming the reactivity of monoterpene synthases to guide regioselective product hydroxylation

Nicole G. H. Leferink, Kara E. Ranaghan, Jaime Battye, Linus O. Johannissen, Sam Hay, Marc W. Van Der Kamp, Adrian J Mulholland, Nigel S Scrutton

Research output: Contribution to journalArticle (Academic Journal)peer-review

11 Citations (Scopus)
192 Downloads (Pure)


Monoterpenoids are industrially important natural products
with applications in the flavours, fragrances, fuels and pharmaceutical
industries. Most monoterpenoids are produced by plants, but recently
two bacterial monoterpene synthases have been identified, including
a cineole synthase (bCinS). Unlike plant cineole synthases, bCinS is
capable of producing nearly pure cineole from geranyl diphosphate in
a complex cyclisation cascade that is tightly controlled. Here we have
used a multidisciplinary approach to show that Asn305 controls water
attack on the α-terpinyl cation and subsequent cyclisation and deprotonation of the α-terpineol intermediate, key steps in the cyclisation
cascade which direct product formation towards cineole. Mutation of
Asn305 results in variants that no longer produce α-terpineol or
cineole. MD simulations revealed that water coordination is disrupted
in all variants tested. QM calculations indicate that Asn305 is most
likely a (transient) proton acceptor for the final deprotonation step. Our
synergistic approach gives unique insights into how a single residue,
Asn305, tames the promiscuous chemistry of monoterpene synthase
cyclisation cascades. It does this by tightly controlling the final steps
in cineole formation catalysed by bCinS to form a single hydroxylated
monoterpene product.
Original languageEnglish
Number of pages7
Publication statusPublished - 3 Dec 2019


  • Enzyme catalysis
  • Protein Engineering
  • Molecular dynamics
  • Synthetic Biology
  • Terpenoids


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