Targeted anion transporter delivery by coiled-coil driven membrane fusion

Nestor Lopez Mora, Azadeh Bahreman, Hennie Valkenier, Hongyu Li, Thomas H. Sharp, David N. Sheppard, Anthony P. Davis, Alexander Kros

Research output: Contribution to journalArticle (Academic Journal)

29 Citations (Scopus)
211 Downloads (Pure)

Abstract

Synthetic anion transporters (anionophores) have potential as biomedical research tools and therapeutics. However, the efficient and specific delivery of these highly lipophilic molecules to a target cell membrane is non-trivial. Here, we investigate the delivery of a powerful anionophore to artificial and cell membranes using a coiled-coil-based delivery system inspired by SNARE membrane fusion proteins. Incorporation of complementary lipopeptides into the lipid membranes of liposomes and cell-sized giant unilamellar vesicles (GUVs) facilitated the delivery of a powerful anionophore into GUVs, where its anion transport activity was monitored in real time by fluorescence microscopy. Similar results were achieved using live cells engineered to express a halide-sensitive fluorophore. We conclude that coiled-coil driven membrane fusion is a highly efficient system to deliver anionophores to target cell membranes.
Original languageEnglish
Pages (from-to)1768-1772
Number of pages5
JournalChemical Science
Volume7
Issue number3
Early online date7 Jan 2016
DOIs
Publication statusPublished - 1 Mar 2016

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