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Targeted anion transporter delivery by coiled-coil driven membrane fusion

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)1768-1772
Number of pages5
JournalChemical Science
Volume7
Issue number3
Early online date7 Jan 2016
DOIs
DateAccepted/In press - 6 Jan 2016
DateE-pub ahead of print - 7 Jan 2016
DatePublished (current) - 1 Mar 2016

Abstract

Synthetic anion transporters (anionophores) have potential as biomedical research tools and therapeutics. However, the efficient and specific delivery of these highly lipophilic molecules to a target cell membrane is non-trivial. Here, we investigate the delivery of a powerful anionophore to artificial and cell membranes using a coiled-coil-based delivery system inspired by SNARE membrane fusion proteins. Incorporation of complementary lipopeptides into the lipid membranes of liposomes and cell-sized giant unilamellar vesicles (GUVs) facilitated the delivery of a powerful anionophore into GUVs, where its anion transport activity was monitored in real time by fluorescence microscopy. Similar results were achieved using live cells engineered to express a halide-sensitive fluorophore. We conclude that coiled-coil driven membrane fusion is a highly efficient system to deliver anionophores to target cell membranes.

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via RSC at http://pubs.rsc.org/en/Content/ArticleLanding/2016/SC/C5SC04282H#!divAbstract. Please refer to any applicable terms of use of the publisher.

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