Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis

Kai F Hoettges, Erin Henslee, Ruth M Torcal Serrano, Rita Jabr, Rula Abdallat, Andrew Beale, Abdul Waheed, Patrizia Camelliti, Chris H Fry, Daan van der Veen, Fatima H Labeed*, Michael Hughes

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

11 Citations (Scopus)
34 Downloads (Pure)

Abstract

Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to measure; gold-standard techniques are typically unable to measure more than a few cells per day, making widespread adoption difficult and limiting statistical reproducibility. We have developed a dielectrophoretic platform using a disposable 3D electrode geometry that accurately (r2>0.99) measures mean electrical properties of populations of ~20,000 cells, by taking parallel ensemble measurements of cells at 20 frequencies up to 45 MHz, in (typically) ten seconds. This allows acquisition of ultra-high-resolution (100-point) DEP spectra in under two minutes. Data acquired from a wide range of cells – from platelets to large cardiac cells - benchmark well with patch-clamp-data. These advantages are collectively demonstrated in a longitudinal (same-animal) study of rapidly-changing phenomena such as ultradian (2-3 hour) rhythmicity in whole blood samples of the common vole (Microtus arvalis), taken from 10 µl tail-nick blood samples and avoiding sacrifice of the animal that is typically required in these studies.
Original languageEnglish
Article number19153 (2019)
Number of pages13
JournalScientific Reports
Volume9
DOIs
Publication statusPublished - 16 Dec 2019

Keywords

  • Dielectrophoresis
  • Electrorotation
  • DEP
  • electrokinetics
  • membrane

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