Abstract
Results: We simulated eight minimal gene sets from the literature, finding that they produced in-silico cells that did not divide. Using knowledge from previous research, we reintroduced specific essential and low essential genes in-silico; this enabled the cells to divide.
Conclusions: This reinforces the need to identify species-specific low essential genes and their interactions. Any genome designs created using the currently incomplete and fragmented gene essentiality information, will very likely require in-vivo reintroductions to correct issues and produce dividing cells.
Original language | English |
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Journal | Genome Biology |
Publication status | Submitted - 10 Sept 2020 |
Structured keywords
- Bristol BioDesign Institute
- BrisSynBio
Keywords
- synthetic biology
Fingerprint
Dive into the research topics of 'Testing theoretical minimal genomes using whole-cell models'. Together they form a unique fingerprint.Student theses
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Minimal Genome Design and Engineering: Algorithms and whole-cell Models
Author: Rees-Garbutt, J. P., 26 Nov 2020Supervisor: Marucci, L. (Supervisor) & Grierson, C. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy (PhD)
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Using and improving whole-cell models to investigate bacterial minimal genomes
Author: Rightmyer, J., 28 Sept 2021Supervisor: Grierson, C. (Supervisor) & Marucci, L. (Supervisor)
Student thesis: Master's Thesis › Master of Science (MSc)
File
Datasets
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Data from minimal gene set whole-cell modelling
Marucci, L. (Creator), Grierson, C. S. (Creator), Chalkley, O. (Creator), Rees-Garbutt, J. P. (Creator) & Rightmyer, J. (Creator), University of Bristol, 30 Apr 2021
DOI: 10.5523/bris.356boayld729v2snyncfoltt0w, http://data.bris.ac.uk/data/dataset/356boayld729v2snyncfoltt0w
Dataset
Equipment
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HPC (High Performance Computing) Facility
Sadaf R Alam (Manager), Steven A Chapman (Manager), Polly E Eccleston (Other), Simon H Atack (Other) & D A G Williams (Manager)
Facility/equipment: Facility