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TFG Promotes Organization of Transitional ER and Efficient Collagen Secretion

Research output: Contribution to journalArticle

Original languageEnglish
Article number15
Pages (from-to)1648-59
Number of pages12
JournalCell Reports
Volume15
Issue number8
Early online date12 May 2016
DOIs
DateAccepted/In press - 15 Apr 2016
DateE-pub ahead of print - 12 May 2016
DatePublished (current) - 24 May 2016

Abstract

Collagen is the most abundant protein in the animal kingdom. It is of fundamental importance during development for cell differentiation and tissue morphogenesis as well as in pathological processes such as fibrosis and cancer cell migration. However, our understanding of the mechanisms of procollagen secretion remains limited. Here, we show that TFG organizes transitional ER (tER) and ER exit sites (ERESs) into larger structures. Depletion of TFG results in dispersion of tER elements that remain associated with individual ER-Golgi intermediate compartments (ERGICs) as largely functional ERESs. We show that TFG is not required for the transport and packaging of small soluble cargoes but is necessary for the export of procollagen from the ER. Our work therefore suggests a key relationship between the structure and function of ERESs and a central role for TFG in optimizing COPII assembly for procollagen export.

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Elsevier (Cell) at http://www.cell.com/cell-reports/fulltext/S2211-1247%2816%2930506-X

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