The alternative pathway C20 Δ8-desaturase from the non-photosynthetic organism Acanthameoba castellanii is an atypical cytochrome b5-fusion desaturase

O Sayanova, R Haslam, B Qi, CM Lazarus, JA Napier

Research output: Contribution to journalArticle (Academic Journal)peer-review

36 Citations (Scopus)

Abstract

A cDNA encoding a C20 Δ8-desaturase was isolated from the free-living soil amoeba, Acanthamoeba castellanii and functionally characterised by heterologous expression. The open reading frame of the A. castellanii C20 Δ8-desaturase showed similarity to other microsomal front-end desaturases, but the N-terminal domain contained a variant form of the conserved heme-binding motif in which H-P-G-G is replaced by H-P-A-G. Co-expression of the A. castellani Δ8-desaturase with the Isochrysis galbana Δ9-elongase in transgenic Arabidopsis plants confirmed the activity observed in yeast and its role in the alternative pathway for C20 polyunsaturated fatty acid synthesis. Acyl-CoA profiles of these transgenic plants revealed an unexpected accumulation of C20 fatty acids in the acyl-CoA pool. This is the first report of an alternative pathway C20 Δ8-desaturase from a non-photosynthetic organism, and also the first report of a front-end desaturase lacking the canonical cytochrome b5 domain.
Translated title of the contributionThe alternative pathway C20 Δ8-desaturase from the non-photosynthetic organism Acanthameoba castellanii is an atypical cytochrome b5-fusion desaturase
Original languageEnglish
Pages (from-to)1946 - 1952
Number of pages9
JournalFEBS Letters
Volume580 (8)
DOIs
Publication statusPublished - Apr 2006

Bibliographical note

Publisher: Elsevier

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