The anxiogenic drug FG-7142 increases serotonin metabolism in the rat medial prefrontal cortex

A Evans, JK Abrams, JA Bouwknecht, DM Knight, A Shekhar, CA Lowry

Research output: Contribution to journalArticle (Academic Journal)

17 Citations (Scopus)

Abstract

The neural mechanisms underlying anxiety states are believed to involve interactions among forebrain limbic circuits and brainstem serotonergic systems. Consistent with this hypothesis, FG-7142, a partial inverse agonist at the benzodiazepine allosteric site of the GABAA receptor, increases c-Fos expression within a subpopulation of brainstem serotonergic neurons. Paradoxically, FG-7142 has no effect on extracellular serotonin concentrations, as measured using in vivo microdialysis, in certain anxiety-related brain structures. This study tested the hypothesis that FG-7142 alters serotonin metabolism within one or more nodes of a defined anxiety-related forebrain circuit. Rats received one of four treatments (vehicle, 1.9, 3.8, or 7.5 mg/kg FG-7142, i.p.) and brains were collected 1 h following treatment. Thirteen forebrain regions were microdissected and analyzed for l-tryptophan, serotonin, and 5-hydroxyindoleacetic acid concentrations using high pressure liquid chromatography with electrochemical detection. FG-7142 (7.5 mg/kg) increased l-tryptophan, serotonin, and 5-hydroxyindoleacetic acid concentrations in the prelimbic cortex but not in several other regions studied including subdivisions of the amygdala and bed nucleus of the stria terminalis. These data demonstrate that FG-7142 alters brain tryptophan concentrations and serotonin metabolism in specific components of an anxiety-related forebrain circuit including the medial prefrontal cortex, an important structure involved in executive function and the regulation of emotional behavior.
Translated title of the contributionThe anxiogenic drug FG-7142 increases serotonin metabolism in the rat medial prefrontal cortex
Original languageEnglish
Pages (from-to)266 - 274
Number of pages9
JournalPharmacology, Biochemistry and Behavior
Volume84 (2)
DOIs
Publication statusPublished - Jun 2006

Bibliographical note

Publisher: Elsevier

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