TY - JOUR
T1 - The Arabidopsis Rab protein RABC1 affects stomatal development by regulating lipid droplet dynamics
AU - Ge, Shengchao
AU - Zhang, Ruo Xi
AU - Wang, Yi Fei
AU - Sun, Pengyue
AU - Chu, Jiaheng
AU - Li, Jiao
AU - Sun, Peng
AU - Wang, Jianbo
AU - Hetherington, Alistair M.
AU - Liang, Yun Kuan
N1 - Publisher Copyright:
© American Society of Plant Biologists 2022. All rights reserved. For permissions, please email: [email protected].
PY - 2022/10/27
Y1 - 2022/10/27
N2 - Lipid droplets (LDs) are evolutionarily conserved organelles that serve as hubs of cellular lipid and energy metabolism in virtually all organisms. Mobilization of LDs is important in light-induced stomatal opening. However, whether and how LDs are involved in stomatal development remains unknown. We show here that Arabidopsis thaliana LIPID DROPLETS AND STOMATA 1 (LDS1)/RABC1 (At1g43890) encodes a member of the Rab GTPase family that is involved in regulating LD dynamics and stomatal morphogenesis. The expression of RABC1 is coordinated with the different phases of stomatal development. RABC1 targets to the surface of LDs in response to oleic acid application in a RABC1GEF1-dependent manner. RABC1 physically interacts with SEIPIN2/3, two orthologues of mammalian seipin, which function in the formation of LDs. Disruption of RABC1, RABC1GEF1, or SEIPIN2/3 resulted in aberrantly large LDs, severe defects in guard cell vacuole morphology, and stomatal function. In conclusion, these findings reveal an aspect of LD function and uncover a role for lipid metabolism in stomatal development in plants.
AB - Lipid droplets (LDs) are evolutionarily conserved organelles that serve as hubs of cellular lipid and energy metabolism in virtually all organisms. Mobilization of LDs is important in light-induced stomatal opening. However, whether and how LDs are involved in stomatal development remains unknown. We show here that Arabidopsis thaliana LIPID DROPLETS AND STOMATA 1 (LDS1)/RABC1 (At1g43890) encodes a member of the Rab GTPase family that is involved in regulating LD dynamics and stomatal morphogenesis. The expression of RABC1 is coordinated with the different phases of stomatal development. RABC1 targets to the surface of LDs in response to oleic acid application in a RABC1GEF1-dependent manner. RABC1 physically interacts with SEIPIN2/3, two orthologues of mammalian seipin, which function in the formation of LDs. Disruption of RABC1, RABC1GEF1, or SEIPIN2/3 resulted in aberrantly large LDs, severe defects in guard cell vacuole morphology, and stomatal function. In conclusion, these findings reveal an aspect of LD function and uncover a role for lipid metabolism in stomatal development in plants.
UR - http://www.scopus.com/inward/record.url?scp=85140862073&partnerID=8YFLogxK
U2 - 10.1093/plcell/koac239
DO - 10.1093/plcell/koac239
M3 - Article (Academic Journal)
C2 - 35929087
SN - 1040-4651
VL - 34
SP - 4274
EP - 4292
JO - The Plant Cell
JF - The Plant Cell
IS - 11
M1 - koac239
ER -