Projects per year
Abstract
We conducted an infrared thermal imaging-based genetic screen to identify Arabidopsis mutants displaying aberrant stomatal behavior in response to elevated concentrations of CO2. This approach resulted in the isolation of a novel allele of the Arabidopsis BIG locus (At3g02260) that we have called CO2 insensitive 1 (cis1). BIG mutants are compromised in elevated CO2-induced stomatal closure and bicarbonate activation of S-type anion channel currents. In contrast with the wild-type, they fail to exhibit reductions in stomatal density and index when grown in elevated CO2. However, like the wild-type, BIG mutants display inhibition of stomatal opening when exposed to elevated CO2. BIG mutants also display wild-type stomatal aperture responses to the closure-inducing stimulus abscisic acid (ABA). Our results indicate that BIG is a signaling component involved in the elevated CO2-mediated control of stomatal development. In the control of stomatal aperture by CO2, BIG is only required in elevated CO2-induced closure and not in the inhibition of stomatal opening by this environmental signal. These data show that, at the molecular level, the CO2-mediated inhibition of opening and promotion of stomatal closure signaling pathways are separable and BIG represents a distinguishing element in these two CO2-mediated responses.
Original language | English |
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Number of pages | 10 |
Journal | New Phytologist |
Early online date | 2 Jan 2018 |
DOIs | |
Publication status | E-pub ahead of print - 2 Jan 2018 |
Keywords
- Abscisic acid (ABA)
- Arabidopsis thaliana
- BIG gene
- CO signaling
- S-type anion channel
- Stomatal function
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Dive into the research topics of 'The BIG protein distinguishes the process of CO2-induced stomatal closure from the inhibition of stomatal opening by CO2'. Together they form a unique fingerprint.Projects
- 2 Finished
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The control of specificity in guard cell ROS-based signalling
Hetherington, A. M. (Principal Investigator)
15/02/16 → 14/02/19
Project: Research
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New insights into the control of stomatal aperture and development by CO2
Hetherington, A. M. (Principal Investigator)
15/01/12 → 15/04/15
Project: Research