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The characterisation of microsatellite markers reveals tetraploidy in the Greater Water Parsnip, Sium latifolium (Apiaceae)

Research output: Contribution to journalArticle

  • Naomi J Dalton
  • Gavin J Horsburgh
  • Deborah A Dawson
Original languageEnglish
Pages (from-to)204
JournalBMC Research Notes
Volume10
Issue number1
DOIs
DateAccepted/In press - 2 Jun 2017
DatePublished (current) - 12 Jun 2017

Abstract

BACKGROUND: The Greater Water Parsnip, Sium latifolium (Apiaceae), is a marginal aquatic perennial currently endangered in England and consequently the focus of a number of conservation translocation projects. Microsatellite markers were developed for S. latifolium to facilitate comparison of genetic diversity and composition between natural and introduced populations.

RESULTS: We selected 65 S. latifolium microsatellite (MiSeq) sequences and designed primer pairs for these. Primer sets were tested in 32 individuals. We found 15 polymorphic loci that amplified consistently. For the selected 15 loci, the number of alleles per locus ranged from 8 to 17. For all loci, S. latifolium individuals displayed up to four alleles indicating polyploidy in this species.

CONCLUSIONS: These are the first microsatellite loci developed for S. latifolium and each individual displayed 1-4 alleles per locus, suggesting polyploidy in this species. These markers provide a valuable resource in evaluating the population genetic composition of this endangered species and thus will be useful for guiding conservation and future translocations of the species.

    Research areas

  • SIUM LATIFOLIUM , Microsatellite, polyploid, Plant translocation, simple sequence repeat (SSR)simple tandem repeat (STR)

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via BioMed Central at https://doi.org/10.1186/s13104-017-2528-6 . Please refer to any applicable terms of use of the publisher.

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    Licence: CC BY

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