The coculture organotypic assay of angiogenesis

Jennifer L Allen; Harry Mellor

doi:10.1007/978-1-4939-1462-3_17

The coculture organotypic assay of angiogenesis

Jennifer L Allen, Harry Mellor

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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Abstract

Angiogenesis is a complex process involving the interactions of endothelial cells not only with pro-angiogenic factors but also with stromal cells and stromal matrix components. Modeling this process in vitro is challenging, and many different assays have been described, each with their own particular strengths and weaknesses. The coculture assay is a long-term assay of angiogenesis that uses fibroblasts to secrete and condition a stromal matrix that more closely mimics tissue than a simple collagen gel. The assay is particularly suited to screening for angiogenic regulators and also for high-resolution imaging of endothelial cells undergoing angiogenic morphogenesis.

Original language	English
Pages (from-to)	265-70
Number of pages	6
Journal	Methods in Molecular Biology
Volume	1214
DOIs	https://doi.org/10.1007/978-1-4939-1462-3_17
Publication status	Published - 2015

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title = "The coculture organotypic assay of angiogenesis",

abstract = "Angiogenesis is a complex process involving the interactions of endothelial cells not only with pro-angiogenic factors but also with stromal cells and stromal matrix components. Modeling this process in vitro is challenging, and many different assays have been described, each with their own particular strengths and weaknesses. The coculture assay is a long-term assay of angiogenesis that uses fibroblasts to secrete and condition a stromal matrix that more closely mimics tissue than a simple collagen gel. The assay is particularly suited to screening for angiogenic regulators and also for high-resolution imaging of endothelial cells undergoing angiogenic morphogenesis.",

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AU - Mellor, Harry

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AB - Angiogenesis is a complex process involving the interactions of endothelial cells not only with pro-angiogenic factors but also with stromal cells and stromal matrix components. Modeling this process in vitro is challenging, and many different assays have been described, each with their own particular strengths and weaknesses. The coculture assay is a long-term assay of angiogenesis that uses fibroblasts to secrete and condition a stromal matrix that more closely mimics tissue than a simple collagen gel. The assay is particularly suited to screening for angiogenic regulators and also for high-resolution imaging of endothelial cells undergoing angiogenic morphogenesis.

U2 - 10.1007/978-1-4939-1462-3_17

DO - 10.1007/978-1-4939-1462-3_17

M3 - Article (Academic Journal)

C2 - 25468611

VL - 1214

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EP - 270

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

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