The effect of omega-3 polyunsaturated fatty acids on the inflammatory response of the amnion

Lorraine Frew; N. U. Sugiarto; Shalini P. Rajagopal; J. He; Rosemary Leask; Jane E. Norman; Simon C. Riley; Sarah J. Stock

doi:10.1016/j.plefa.2013.07.001

The effect of omega-3 polyunsaturated fatty acids on the inflammatory response of the amnion

Lorraine Frew, N. U. Sugiarto, Shalini P. Rajagopal, J. He, Rosemary Leask, Jane E. Norman, Simon C. Riley, Sarah J. Stock

Health Sciences Faculty Office

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Abstract

ObjectiveTo investigate the effect of omega-3 PUFAs, eicosapentanoic acid (EPA) and docosohexanoic acid (DHA) on inflammatory cytokine production in the amnion.Study designAmnion explants were obtained at elective caesarean sections and cultured in vitro with EPA and DHA. IL-8 and IL-6 secretion was determined by ELISA, the role of PPARγ was investigated using specific agonists and antagonists and activity of MMP assessed by gelatin zymography.ResultsA combination of EPA and DHA significantly reduced the concentration of IL-8 and IL-6 released into the supernatant compared to untreated controls (p

Original language	English
Pages (from-to)	221-225
Number of pages	5
Journal	Prostaglandins, Leukotrienes, and Essential Fatty Acids
Volume	89
Issue number	4
DOIs	https://doi.org/10.1016/j.plefa.2013.07.001
Publication status	Published - 2013

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title = "The effect of omega-3 polyunsaturated fatty acids on the inflammatory response of the amnion",

abstract = "ObjectiveTo investigate the effect of omega-3 PUFAs, eicosapentanoic acid (EPA) and docosohexanoic acid (DHA) on inflammatory cytokine production in the amnion.Study designAmnion explants were obtained at elective caesarean sections and cultured in vitro with EPA and DHA. IL-8 and IL-6 secretion was determined by ELISA, the role of PPARγ was investigated using specific agonists and antagonists and activity of MMP assessed by gelatin zymography.ResultsA combination of EPA and DHA significantly reduced the concentration of IL-8 and IL-6 released into the supernatant compared to untreated controls (p",

author = "Lorraine Frew and Sugiarto, {N. U.} and Rajagopal, {Shalini P.} and J. He and Rosemary Leask and Norman, {Jane E.} and Riley, {Simon C.} and Stock, {Sarah J.}",

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language = "English",

volume = "89",

pages = "221--225",

journal = "Prostaglandins, Leukotrienes, and Essential Fatty Acids",

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TY - JOUR

T1 - The effect of omega-3 polyunsaturated fatty acids on the inflammatory response of the amnion

AU - Frew, Lorraine

AU - Sugiarto, N. U.

AU - Rajagopal, Shalini P.

AU - He, J.

AU - Leask, Rosemary

AU - Norman, Jane E.

AU - Riley, Simon C.

AU - Stock, Sarah J.

N1 - RIS file

PY - 2013

Y1 - 2013

N2 - ObjectiveTo investigate the effect of omega-3 PUFAs, eicosapentanoic acid (EPA) and docosohexanoic acid (DHA) on inflammatory cytokine production in the amnion.Study designAmnion explants were obtained at elective caesarean sections and cultured in vitro with EPA and DHA. IL-8 and IL-6 secretion was determined by ELISA, the role of PPARγ was investigated using specific agonists and antagonists and activity of MMP assessed by gelatin zymography.ResultsA combination of EPA and DHA significantly reduced the concentration of IL-8 and IL-6 released into the supernatant compared to untreated controls (p

AB - ObjectiveTo investigate the effect of omega-3 PUFAs, eicosapentanoic acid (EPA) and docosohexanoic acid (DHA) on inflammatory cytokine production in the amnion.Study designAmnion explants were obtained at elective caesarean sections and cultured in vitro with EPA and DHA. IL-8 and IL-6 secretion was determined by ELISA, the role of PPARγ was investigated using specific agonists and antagonists and activity of MMP assessed by gelatin zymography.ResultsA combination of EPA and DHA significantly reduced the concentration of IL-8 and IL-6 released into the supernatant compared to untreated controls (p

U2 - 10.1016/j.plefa.2013.07.001

DO - 10.1016/j.plefa.2013.07.001

M3 - Article (Academic Journal)

C2 - 23890848

SN - 0952-3278

VL - 89

SP - 221

EP - 225

JO - Prostaglandins, Leukotrienes, and Essential Fatty Acids

JF - Prostaglandins, Leukotrienes, and Essential Fatty Acids

IS - 4

ER -

The effect of omega-3 polyunsaturated fatty acids on the inflammatory response of the amnion

Abstract

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