The effect of polyamines on KATP channels in guinea-pig ventricular myocytes

X W Niu; R W Meech

doi:10.1111/j.1469-7793.1998.401bq.x

The effect of polyamines on K_ATP channels in guinea-pig ventricular myocytes

X W Niu
, R W Meech

Research output: Contribution to journal › Article (Academic Journal) › peer-review

10 Citations (Scopus)

Abstract

1. The effect of natural polyamines on K_ATP channels was studied using inside-out patches from guinea-pig ventricular myocytes.

2. At a holding potential of +40 mV, spermine at the intracellular membrane surface reduced the K_ATP channel open probability (Popen) in a dose-dependent manner. Half-maximal inhibition occurred at 29 microM with a Hill coefficient of 1.2.

3. The effect of spermine on Popen was not greatly influenced by the membrane potential but there appeared to be a small reduction in unitary current amplitude during strong depolarizations.

4. Analysis of K_ATP single channel kinetics showed that spermine inhibited the channel by decreasing the mean open time and introducing transitions to a long closed state.

5. Spermidine (0.1 mM) was found to have a similar effect to spermine. Putrescine (10 mM) was found to block more effectively at positive membrane potentials. Up to 20 mM arginine had no significant effect on K_ATP channels.

6. Our results indicate that natural polyamines influence native K_ATP channel gating in cardiac myocytes.

Translated title of the contribution	The effect of polyamines on K_A_T_P channels in guinea-pig ventricular myocytes
Original language	English
Pages (from-to)	401-11
Number of pages	11
Journal	Journal of Physiology
Volume	508
Issue number	2
DOIs	https://doi.org/10.1111/j.1469-7793.1998.401bq.x
Publication status	Published - 15 Apr 1998

Keywords

Adenosine Triphosphate
Algorithms
Animals
Arginine
Biogenic Polyamines
Electrophysiology
Guinea Pigs
Heart Ventricles
In Vitro Techniques
Kinetics
Magnesium
Membrane Potentials
Myocardium
Patch-Clamp Techniques
Potassium Channel Blockers
Potassium Channels

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@article{be03d5d3d03945e6bf920719269179f2,

title = "The effect of polyamines on KATP channels in guinea-pig ventricular myocytes",

abstract = "1. The effect of natural polyamines on KATP channels was studied using inside-out patches from guinea-pig ventricular myocytes.2. At a holding potential of +40 mV, spermine at the intracellular membrane surface reduced the KATP channel open probability (Popen) in a dose-dependent manner. Half-maximal inhibition occurred at 29 microM with a Hill coefficient of 1.2.3. The effect of spermine on Popen was not greatly influenced by the membrane potential but there appeared to be a small reduction in unitary current amplitude during strong depolarizations.4. Analysis of KATP single channel kinetics showed that spermine inhibited the channel by decreasing the mean open time and introducing transitions to a long closed state.5. Spermidine (0.1 mM) was found to have a similar effect to spermine. Putrescine (10 mM) was found to block more effectively at positive membrane potentials. Up to 20 mM arginine had no significant effect on KATP channels.6. Our results indicate that natural polyamines influence native KATP channel gating in cardiac myocytes.",

keywords = "Adenosine Triphosphate, Algorithms, Animals, Arginine, Biogenic Polyamines, Electrophysiology, Guinea Pigs, Heart Ventricles, In Vitro Techniques, Kinetics, Magnesium, Membrane Potentials, Myocardium, Patch-Clamp Techniques, Potassium Channel Blockers, Potassium Channels",

author = "Niu, \{X W\} and Meech, \{R W\}",

year = "1998",

month = apr,

day = "15",

doi = "10.1111/j.1469-7793.1998.401bq.x",

language = "English",

volume = "508",

pages = "401--11",

journal = "Journal of Physiology",

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TY - JOUR

T1 - The effect of polyamines on KATP channels in guinea-pig ventricular myocytes

AU - Niu, X W

AU - Meech, R W

PY - 1998/4/15

Y1 - 1998/4/15

N2 - 1. The effect of natural polyamines on KATP channels was studied using inside-out patches from guinea-pig ventricular myocytes.2. At a holding potential of +40 mV, spermine at the intracellular membrane surface reduced the KATP channel open probability (Popen) in a dose-dependent manner. Half-maximal inhibition occurred at 29 microM with a Hill coefficient of 1.2.3. The effect of spermine on Popen was not greatly influenced by the membrane potential but there appeared to be a small reduction in unitary current amplitude during strong depolarizations.4. Analysis of KATP single channel kinetics showed that spermine inhibited the channel by decreasing the mean open time and introducing transitions to a long closed state.5. Spermidine (0.1 mM) was found to have a similar effect to spermine. Putrescine (10 mM) was found to block more effectively at positive membrane potentials. Up to 20 mM arginine had no significant effect on KATP channels.6. Our results indicate that natural polyamines influence native KATP channel gating in cardiac myocytes.

AB - 1. The effect of natural polyamines on KATP channels was studied using inside-out patches from guinea-pig ventricular myocytes.2. At a holding potential of +40 mV, spermine at the intracellular membrane surface reduced the KATP channel open probability (Popen) in a dose-dependent manner. Half-maximal inhibition occurred at 29 microM with a Hill coefficient of 1.2.3. The effect of spermine on Popen was not greatly influenced by the membrane potential but there appeared to be a small reduction in unitary current amplitude during strong depolarizations.4. Analysis of KATP single channel kinetics showed that spermine inhibited the channel by decreasing the mean open time and introducing transitions to a long closed state.5. Spermidine (0.1 mM) was found to have a similar effect to spermine. Putrescine (10 mM) was found to block more effectively at positive membrane potentials. Up to 20 mM arginine had no significant effect on KATP channels.6. Our results indicate that natural polyamines influence native KATP channel gating in cardiac myocytes.

KW - Adenosine Triphosphate

KW - Algorithms

KW - Animals

KW - Arginine

KW - Biogenic Polyamines

KW - Electrophysiology

KW - Guinea Pigs

KW - Heart Ventricles

KW - In Vitro Techniques

KW - Kinetics

KW - Magnesium

KW - Membrane Potentials

KW - Myocardium

KW - Patch-Clamp Techniques

KW - Potassium Channel Blockers

KW - Potassium Channels

U2 - 10.1111/j.1469-7793.1998.401bq.x

DO - 10.1111/j.1469-7793.1998.401bq.x

M3 - Article (Academic Journal)

C2 - 9508805

SN - 0022-3751

VL - 508

SP - 401

EP - 411

JO - Journal of Physiology

JF - Journal of Physiology

IS - 2

ER -