The effect of protease inhibition on the temporal stability of NT-proBNP in feline plasma at room temperature

David J Connolly; Melanie J Hezzell; Virginia Luis Fuentes; Yu-Mei Chang; Rebecca Swan; Harriet M Syme

doi:10.1016/j.jvc.2010.11.002

The effect of protease inhibition on the temporal stability of NT-proBNP in feline plasma at room temperature

David J Connolly, Melanie J Hezzell, Virginia Luis Fuentes, Yu-Mei Chang, Rebecca Swan, Harriet M Syme

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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Abstract

OBJECTIVES: To evaluate whether the addition of a protease inhibitor (PI) to feline plasma improves the temporal stability of N-terminal pro-B-type natriuretic peptide (NT-proBNP).

ANIMALS, MATERIALS AND METHODS: Forty-four EDTA blood samples were collected from 42 cats with cardiac disease or hyperthyroidism. Samples were separated within 15 min of collection and the plasma stored at -80 °C. Samples were thawed and each separated into 2 aliquots, one of which was mixed with PI. Each was subdivided into 5 aliquots, which remained at room temperature (RT) for zero, 24, 48, 96 or 120 h before being returned to storage at -80 °C. NT-proBNP was measured using a commercially-available ELISA.

RESULTS: There was no difference in NT-proBNP measurements between plain and PI samples at time zero (P = 0.836) or 24 h (P = 0.293). At subsequent time-points NT-proBNP was higher in the PI than the plain samples (P < 0.05). An overall effect of time at RT was detected for plain (P < 0.001) and PI samples (P < 0.001).

CONCLUSIONS: The addition of PI to feline plasma significantly reduces, but does not eliminate, degradation of NT-proBNP at RT. This degradation occurs over a time course comparable to postal transportation of samples for laboratory analysis and may alter the clinical interpretation of results.

Original language	English
Pages (from-to)	13-9
Number of pages	7
Journal	Journal of Veterinary Cardiology
Volume	13
Issue number	1
DOIs	https://doi.org/10.1016/j.jvc.2010.11.002
Publication status	Published - Mar 2011

Keywords

Animals
Cats
Natriuretic Peptide, Brain
Peptide Fragments
Protease Inhibitors
Specimen Handling
Temperature
Time Factors

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@article{c419b8cea194411dbba5a5e16e0f2c0b,

title = "The effect of protease inhibition on the temporal stability of NT-proBNP in feline plasma at room temperature",

abstract = "OBJECTIVES: To evaluate whether the addition of a protease inhibitor (PI) to feline plasma improves the temporal stability of N-terminal pro-B-type natriuretic peptide (NT-proBNP).ANIMALS, MATERIALS AND METHODS: Forty-four EDTA blood samples were collected from 42 cats with cardiac disease or hyperthyroidism. Samples were separated within 15 min of collection and the plasma stored at -80 °C. Samples were thawed and each separated into 2 aliquots, one of which was mixed with PI. Each was subdivided into 5 aliquots, which remained at room temperature (RT) for zero, 24, 48, 96 or 120 h before being returned to storage at -80 °C. NT-proBNP was measured using a commercially-available ELISA.RESULTS: There was no difference in NT-proBNP measurements between plain and PI samples at time zero (P = 0.836) or 24 h (P = 0.293). At subsequent time-points NT-proBNP was higher in the PI than the plain samples (P < 0.05). An overall effect of time at RT was detected for plain (P < 0.001) and PI samples (P < 0.001).CONCLUSIONS: The addition of PI to feline plasma significantly reduces, but does not eliminate, degradation of NT-proBNP at RT. This degradation occurs over a time course comparable to postal transportation of samples for laboratory analysis and may alter the clinical interpretation of results.",

keywords = "Animals, Cats, Natriuretic Peptide, Brain, Peptide Fragments, Protease Inhibitors, Specimen Handling, Temperature, Time Factors",

author = "Connolly, {David J} and Hezzell, {Melanie J} and Fuentes, {Virginia Luis} and Yu-Mei Chang and Rebecca Swan and Syme, {Harriet M}",

year = "2011",

month = mar,

doi = "10.1016/j.jvc.2010.11.002",

language = "English",

volume = "13",

pages = "13--9",

journal = "Journal of Veterinary Cardiology",

issn = "1875-0834",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - The effect of protease inhibition on the temporal stability of NT-proBNP in feline plasma at room temperature

AU - Connolly, David J

AU - Hezzell, Melanie J

AU - Fuentes, Virginia Luis

AU - Chang, Yu-Mei

AU - Swan, Rebecca

AU - Syme, Harriet M

PY - 2011/3

Y1 - 2011/3

N2 - OBJECTIVES: To evaluate whether the addition of a protease inhibitor (PI) to feline plasma improves the temporal stability of N-terminal pro-B-type natriuretic peptide (NT-proBNP).ANIMALS, MATERIALS AND METHODS: Forty-four EDTA blood samples were collected from 42 cats with cardiac disease or hyperthyroidism. Samples were separated within 15 min of collection and the plasma stored at -80 °C. Samples were thawed and each separated into 2 aliquots, one of which was mixed with PI. Each was subdivided into 5 aliquots, which remained at room temperature (RT) for zero, 24, 48, 96 or 120 h before being returned to storage at -80 °C. NT-proBNP was measured using a commercially-available ELISA.RESULTS: There was no difference in NT-proBNP measurements between plain and PI samples at time zero (P = 0.836) or 24 h (P = 0.293). At subsequent time-points NT-proBNP was higher in the PI than the plain samples (P < 0.05). An overall effect of time at RT was detected for plain (P < 0.001) and PI samples (P < 0.001).CONCLUSIONS: The addition of PI to feline plasma significantly reduces, but does not eliminate, degradation of NT-proBNP at RT. This degradation occurs over a time course comparable to postal transportation of samples for laboratory analysis and may alter the clinical interpretation of results.

AB - OBJECTIVES: To evaluate whether the addition of a protease inhibitor (PI) to feline plasma improves the temporal stability of N-terminal pro-B-type natriuretic peptide (NT-proBNP).ANIMALS, MATERIALS AND METHODS: Forty-four EDTA blood samples were collected from 42 cats with cardiac disease or hyperthyroidism. Samples were separated within 15 min of collection and the plasma stored at -80 °C. Samples were thawed and each separated into 2 aliquots, one of which was mixed with PI. Each was subdivided into 5 aliquots, which remained at room temperature (RT) for zero, 24, 48, 96 or 120 h before being returned to storage at -80 °C. NT-proBNP was measured using a commercially-available ELISA.RESULTS: There was no difference in NT-proBNP measurements between plain and PI samples at time zero (P = 0.836) or 24 h (P = 0.293). At subsequent time-points NT-proBNP was higher in the PI than the plain samples (P < 0.05). An overall effect of time at RT was detected for plain (P < 0.001) and PI samples (P < 0.001).CONCLUSIONS: The addition of PI to feline plasma significantly reduces, but does not eliminate, degradation of NT-proBNP at RT. This degradation occurs over a time course comparable to postal transportation of samples for laboratory analysis and may alter the clinical interpretation of results.

KW - Animals

KW - Cats

KW - Natriuretic Peptide, Brain

KW - Peptide Fragments

KW - Protease Inhibitors

KW - Specimen Handling

KW - Temperature

KW - Time Factors

U2 - 10.1016/j.jvc.2010.11.002

DO - 10.1016/j.jvc.2010.11.002

M3 - Article (Academic Journal)

C2 - 21296042

SN - 1875-0834

VL - 13

SP - 13

EP - 19

JO - Journal of Veterinary Cardiology

JF - Journal of Veterinary Cardiology

IS - 1

ER -

The effect of protease inhibition on the temporal stability of NT-proBNP in feline plasma at room temperature

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