The effect of resuscitation in 100% oxygen on brain injury in a newborn rat model of severe hypoxic-ischaemic encephalopathy

Elisa Smit, Xun Liu, Hannah Gill, Sally Jary, Thomas Wood, Marianne Thoresen

Research output: Contribution to journalArticle (Academic Journal)

6 Citations (Scopus)
365 Downloads (Pure)

Abstract

AIM: Infants with birth asphyxia frequently require resuscitation. Current guidance is to start newborn resuscitation in 21% oxygen. However, infants with severe hypoxia-ischaemia may require prolonged resuscitation with oxygen. To date, no study has looked at the effect of resuscitation in 100% oxygen following a severe hypoxic-ischaemic insult.

METHODS: Postnatal day 7 Wistar rats underwent a severe hypoxic-ischaemic insult (modified Vannucci unilateral brain injury model) followed by immediate resuscitation in either 21% or 100% oxygen for 30min. Seven days following the insult, negative geotaxis testing was performed in survivors, and the brains were harvested. Relative ipsilateral cortical and hippocampal area loss was assessed histologically.

RESULTS: Total area loss in the affected hemisphere and area loss within the hippocampus did not significantly differ between the two groups. The same results were seen for short-term neurological assessment. No difference was seen in weight gain between pups resuscitated in 21% and 100% oxygen.

CONCLUSION: Resuscitation in 100% oxygen does not cause a deleterious effect on brain injury following a severe hypoxic-ischaemic insult in a rat model of hypoxia-ischaemia. Further work investigating the effects of resuscitation in 100% oxygen is warranted, especially for newborn infants with severe hypoxic-ischaemic encephalopathy.

Original languageEnglish
Pages (from-to)214-9
Number of pages6
JournalResuscitation
Volume96
Early online date21 Aug 2015
DOIs
Publication statusPublished - 1 Nov 2015

Keywords

  • Hyperoxia
  • Oxidative stress
  • Asphyxia
  • Rat model
  • Newborn resuscitation

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