The effect of surface charge on the thermal stability and ice recrystallization inhibition activity of antifreeze protein III (AFP III)

Robert Deller; Ben Carter; Ioannis Zampetakis; Fabrizio Scarpa; Adam Perriman

doi:10.1016/j.bbrc.2017.11.073

The effect of surface charge on the thermal stability and ice recrystallization inhibition activity of antifreeze protein III (AFP III)

Robert Deller, Ben Carter, Ioannis Zampetakis, Fabrizio Scarpa, Adam Perriman^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

12 Citations (Scopus)

445 Downloads (Pure)

Abstract

The aim of this study was to examine the effect of chemical cationization on the structure and function of antifreeze protein III (AFP III) over an extreme temperature range (−40°C to +90°C) using far-UV synchrotron radiation circular dichroism (SRCD) and ice recrystallization inhibition (IRI) assays. Chemical cationization was able to produce a modified AFP III with a net cationic charge at physiological pH that had enhanced resistance to denaturation at elevated temperatures, with no immediate negative impact on protein structure at subzero temperatures. Furthermore, cationized AFP III retained an IRI activity similar to that of native AFP III. Consequently, chemical cationization may provide a pathway to the development of more robust antifreeze proteins as supplementary cryoprotectants in the cryopreservation of clinically relevant cells.

Original language	English
Pages (from-to)	1055-1060
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	495
Issue number	1
Early online date	11 Nov 2017
DOIs	https://doi.org/10.1016/j.bbrc.2017.11.073
Publication status	Published - 1 Jan 2018

Research Groups and Themes

Bristol BioDesign Institute

Keywords

synthetic biology
Ice recrystallization
Cryopreservation
Thermal stability
Synchrotron radiation circular dichroism
Antifreeze protein

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title = "The effect of surface charge on the thermal stability and ice recrystallization inhibition activity of antifreeze protein III (AFP III)",

abstract = "The aim of this study was to examine the effect of chemical cationization on the structure and function of antifreeze protein III (AFP III) over an extreme temperature range (−40°C to +90°C) using far-UV synchrotron radiation circular dichroism (SRCD) and ice recrystallization inhibition (IRI) assays. Chemical cationization was able to produce a modified AFP III with a net cationic charge at physiological pH that had enhanced resistance to denaturation at elevated temperatures, with no immediate negative impact on protein structure at subzero temperatures. Furthermore, cationized AFP III retained an IRI activity similar to that of native AFP III. Consequently, chemical cationization may provide a pathway to the development of more robust antifreeze proteins as supplementary cryoprotectants in the cryopreservation of clinically relevant cells.",

keywords = "synthetic biology, Ice recrystallization, Cryopreservation, Thermal stability, Synchrotron radiation circular dichroism, Antifreeze protein",

author = "Robert Deller and Ben Carter and Ioannis Zampetakis and Fabrizio Scarpa and Adam Perriman",

year = "2018",

month = jan,

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doi = "10.1016/j.bbrc.2017.11.073",

language = "English",

volume = "495",

pages = "1055--1060",

journal = "Biochemical and Biophysical Research Communications",

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publisher = "Academic Press",

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The effect of surface charge on the thermal stability and ice recrystallization inhibition activity of antifreeze protein III (AFP III). / Deller, Robert; Carter, Ben; Zampetakis, Ioannis et al.
In: Biochemical and Biophysical Research Communications, Vol. 495, No. 1, 01.01.2018, p. 1055-1060.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

TY - JOUR

T1 - The effect of surface charge on the thermal stability and ice recrystallization inhibition activity of antifreeze protein III (AFP III)

AU - Deller, Robert

AU - Carter, Ben

AU - Zampetakis, Ioannis

AU - Scarpa, Fabrizio

AU - Perriman, Adam

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The aim of this study was to examine the effect of chemical cationization on the structure and function of antifreeze protein III (AFP III) over an extreme temperature range (−40°C to +90°C) using far-UV synchrotron radiation circular dichroism (SRCD) and ice recrystallization inhibition (IRI) assays. Chemical cationization was able to produce a modified AFP III with a net cationic charge at physiological pH that had enhanced resistance to denaturation at elevated temperatures, with no immediate negative impact on protein structure at subzero temperatures. Furthermore, cationized AFP III retained an IRI activity similar to that of native AFP III. Consequently, chemical cationization may provide a pathway to the development of more robust antifreeze proteins as supplementary cryoprotectants in the cryopreservation of clinically relevant cells.

AB - The aim of this study was to examine the effect of chemical cationization on the structure and function of antifreeze protein III (AFP III) over an extreme temperature range (−40°C to +90°C) using far-UV synchrotron radiation circular dichroism (SRCD) and ice recrystallization inhibition (IRI) assays. Chemical cationization was able to produce a modified AFP III with a net cationic charge at physiological pH that had enhanced resistance to denaturation at elevated temperatures, with no immediate negative impact on protein structure at subzero temperatures. Furthermore, cationized AFP III retained an IRI activity similar to that of native AFP III. Consequently, chemical cationization may provide a pathway to the development of more robust antifreeze proteins as supplementary cryoprotectants in the cryopreservation of clinically relevant cells.

KW - synthetic biology

KW - Ice recrystallization

KW - Cryopreservation

KW - Thermal stability

KW - Synchrotron radiation circular dichroism

KW - Antifreeze protein

UR - http://www.scopus.com/inward/record.url?scp=85034967155&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2017.11.073

DO - 10.1016/j.bbrc.2017.11.073

M3 - Article (Academic Journal)

C2 - 29137985

AN - SCOPUS:85034967155

SN - 0006-291X

VL - 495

SP - 1055

EP - 1060

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

The effect of surface charge on the thermal stability and ice recrystallization inhibition activity of antifreeze protein III (AFP III)

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Functional Biomolecular Liquids

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The effect of surface charge on the thermal stability and ice recrystallization inhibition activity of antifreeze protein III (AFP III)

Abstract

Research Groups and Themes

Keywords

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