The impact of stress on cytokine and haptoglobin mRNA expression in blood samples from harbour porpoises (Phocoena phocoena)

S. Fonfara, U. Siebert, A. Prange, F. Colijn

    Research output: Contribution to journalArticle (Academic Journal)peer-review

    14 Citations (Scopus)

    Abstract

    Cytokines are important cell mediators involved in immune responses. Their expression can be modulated by numerous factors, including stress. The aim of this study was to compare cytokine mRNA expression from harbour porpoises exposed to different environments. Blood samples were taken from two healthy porpoises living in captivity at the Fjord and Belt Centre Kerteminde, Denmark, and from four wild porpoises accidentally caught in Danish waters. Real-time RT-PCR was used to quantify the transcription of interleukin-(IL)-1 beta, IL-2, -4, -6, -10, tumour necrosis factor-(TNF)-alpha, transforming growth factor-(TGF)-beta and the acute phase protcin haptoglobin. This revealed downregulation of the pro-inflammatory cyokines IL-1 beta, IL-2, IL-6 and TNF alpha, and a switch towards the T-helper-cell-(Th)2-and Th3-cytokines, IL-4 and TGF beta, in blood samples of the wild-captured animals. This indicated a shift towards immunomodulatory cytokines. In addition, cortisol levels were increased in the wild-caught porpoises. These results are suggestive of stress-induced modulation of the immune responses in the accidentally caught animals. The current study indicates that the expression pattern of these cytokines and the estimation of the Th1- to Th2- and Th3-cytokine mRNA ratios might be a useful indicator to analyse the influence of stress on the immune system in harbour porpoises.

    Original languageEnglish
    Pages (from-to)305-311
    Number of pages7
    JournalJournal of the Marine Biological Association of the UK
    Volume87
    Issue number1
    DOIs
    Publication statusPublished - Feb 2007

    Cite this