Abstract
Little is known about the role of myocardial inflammation and remodeling in cardiac dysfunction, in particular in association with systemic diseases and aging.
Myocardial samples (interventricular septum, right and left atrium and ventricle) of 20 cats with systemic diseases (group 1), 10 cats with FIP (group 2), 7 cats with cardiac diseases (group 3), and 8 cats without systemic or cardiac diseases (group 4) were collected. Cats from groups 1 and 4 were additionally separated into age groups (<5, 5-10, >10-15 and >15 years of age). Transcription levels of feline interleukin (IL)-1, IL-2, IL-4, IL-6, IL-18, tumour necrosis factor-α (TNF), interferon-γ (IFN), transforming growth factor-β (TGF), matrix metalloproteinase (MMP)-2, MMP-3, MMP-13, tissue inhibitors of metalloproteinase (TIMP)-1, TIMP-2, TIMP-3 were analysed in relation to the house keeping gene GAPDH, using real time RT-PCR.
Transcription of IL-4, IFN, MMP-2, MMP-3, MMP-13 was highest in group 2 (p<0.038), and of IL-1, IL-6, TNF, TGF, TIMP-1, TIMP-3 in groups 2 and 3 (p<0.001). IL-18 and TIMP-2 mRNA concentrations were not different between groups 1, 2, and 3, but higher than in group 4 (p<0.016). Regional differences in mRNA expression were detected in cats of groups 1, 2 and 4. Young cats showed higher transcription levels for IL-1, IL-2, IL-4, IL-6, MMP-3 (p<0.003), whereas TGF increased with age (p=0.036).
Myocardial mRNA expression of markers for inflammation and remodelling was similar in cats with systemic and cardiac diseases, which suggests an impact of systemic diseases on cardiac function. Furthermore, age might influence cardiac inflammation and remodelling.
Myocardial samples (interventricular septum, right and left atrium and ventricle) of 20 cats with systemic diseases (group 1), 10 cats with FIP (group 2), 7 cats with cardiac diseases (group 3), and 8 cats without systemic or cardiac diseases (group 4) were collected. Cats from groups 1 and 4 were additionally separated into age groups (<5, 5-10, >10-15 and >15 years of age). Transcription levels of feline interleukin (IL)-1, IL-2, IL-4, IL-6, IL-18, tumour necrosis factor-α (TNF), interferon-γ (IFN), transforming growth factor-β (TGF), matrix metalloproteinase (MMP)-2, MMP-3, MMP-13, tissue inhibitors of metalloproteinase (TIMP)-1, TIMP-2, TIMP-3 were analysed in relation to the house keeping gene GAPDH, using real time RT-PCR.
Transcription of IL-4, IFN, MMP-2, MMP-3, MMP-13 was highest in group 2 (p<0.038), and of IL-1, IL-6, TNF, TGF, TIMP-1, TIMP-3 in groups 2 and 3 (p<0.001). IL-18 and TIMP-2 mRNA concentrations were not different between groups 1, 2, and 3, but higher than in group 4 (p<0.016). Regional differences in mRNA expression were detected in cats of groups 1, 2 and 4. Young cats showed higher transcription levels for IL-1, IL-2, IL-4, IL-6, MMP-3 (p<0.003), whereas TGF increased with age (p=0.036).
Myocardial mRNA expression of markers for inflammation and remodelling was similar in cats with systemic and cardiac diseases, which suggests an impact of systemic diseases on cardiac function. Furthermore, age might influence cardiac inflammation and remodelling.
| Original language | English |
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| Publication status | Published - Jun 2014 |
| Event | ACVIM - New Orleans, United States Duration: 30 May 2012 → … |
Conference
| Conference | ACVIM |
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| Country | United States |
| City | New Orleans |
| Period | 30/05/12 → … |