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The influence of folate supplementation on global gene expression in normal colonic mucosa of subjects with colorectal adenoma

Research output: Contribution to journalArticle

  • Maria Pufulete
  • Reyad Al-Ghnaniem Abbadi
  • Matthew Arno
  • Mark Ewins
  • Caroline Green
  • Estibaliz Aldecoa-Otalora Astarloa
  • Tom Sanders
  • Peter Emery
Original languageEnglish
Pages (from-to)709-20
Number of pages12
JournalMolecular Nutrition and Food Research
Volume57
Issue number4
DOIs
DatePublished - Apr 2013

Abstract

SCOPE: We determined whether short-term supplementation with a physiological dose of folate alters global gene expression in the normal colonic mucosa of subjects with colorectal adenoma.

METHODS AND RESULTS: Fourteen subjects with adenoma, randomised to receive folic acid (400μg/day, n = 6) or placebo (n = 8) for 10 weeks, had blood samples and colonic tissue biopsies collected before and after the intervention. RNA extracted from colonic tissue samples was used to determine global gene expression in the colon using Affymetrix® Microarray GeneChips and real-time RT-PCR. Following intervention, 67 genes were upregulated and 13 genes were downregulated in the folic acid group, while 21 genes were upregulated and none were downregulated in the placebo group (p < 0.05, adjusted for multiple testing). Thirty-six genes were upregulated and 18 genes were downregulated in the folic acid group when compared with placebo, but none of these were statistically significant after adjustment for multiple testing. These genes are involved in multiple pathways, including cell cycle, signal transduction, cell differentiation, transport, cell division, cell motility, protein transport, and immune response.

CONCLUSION: These results indicate that while folic acid can modify gene expression, it is difficult to separate its effects from the natural variability in gene expression in the colon.

Additional information

© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

    Research areas

  • Aged, Cell Division, Colon, Colorectal Neoplasms, Dietary Supplements, Down-Regulation, Female, Folic Acid, Gene Expression, Humans, Intestinal Mucosa, Male, Middle Aged, Oligonucleotide Array Sequence Analysis, Real-Time Polymerase Chain Reaction, Reproducibility of Results, Up-Regulation

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