The majority of the in vitro erythroid expansion potential resides in CD34- cells, outweighing the contribution of CD34+ cells and significantly increasing the erythroblast yield from peripheral blood samples

E van den Akker; TJ Satchwell; S Pellegrin; G Daniels; AM Toye

doi:10.3324/haematol.2009.019828

The majority of the in vitro erythroid expansion potential resides in CD34^- cells, outweighing the contribution of CD34⁺ cells and significantly increasing the erythroblast yield from peripheral blood samples

E van den Akker
, TJ Satchwell
, S Pellegrin
, G Daniels
, AM Toye

Research output: Contribution to journal › Article (Academic Journal) › peer-review

122 Citations (Scopus)

Abstract

The study of human erythropoiesis in health and disease requires a robust culture system that consistently and reliably generates large numbers of immature erythroblasts that can be induced to differentiate synchronously. We describe a culture method modified from Leberbauer et al. (2005) and obtain a homogenous population of erythroblasts from peripheral blood mononuclear cells (PBMC) without prior purification of CD34+ cells. This pure population of immature erythroblasts can be expanded to obtain 4x10⁸ erythroblasts from 1x10⁸ PBMC after 13-14 days in culture. Upon synchronized differentiation, high levels of enucleation (80-90%) and low levels of cell death (

Translated title of the contribution	The majority of the in vitro erythroid expansion potential resides in CD34^- cells, outweighing the contribution of CD34⁺ cells and significantly increasing the erythroblast yield from peripheral blood samples
Original language	English
Pages (from-to)	1594 - 1598
Number of pages	5
Journal	Haematologica
Volume	95
Issue number	9
DOIs	https://doi.org/10.3324/haematol.2009.019828
Publication status	Published - 1 Sept 2010

Bibliographical note

Other: Advanced online publication April 7, 2010

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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van den Akker, E., Satchwell, TJ., Pellegrin, S., Daniels, G., & Toye, AM. (2010). The majority of the in vitro erythroid expansion potential resides in CD34^- cells, outweighing the contribution of CD34⁺ cells and significantly increasing the erythroblast yield from peripheral blood samples. Haematologica, 95(9), 1594 - 1598. https://doi.org/10.3324/haematol.2009.019828

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abstract = "The study of human erythropoiesis in health and disease requires a robust culture system that consistently and reliably generates large numbers of immature erythroblasts that can be induced to differentiate synchronously. We describe a culture method modified from Leberbauer et al. (2005) and obtain a homogenous population of erythroblasts from peripheral blood mononuclear cells (PBMC) without prior purification of CD34+ cells. This pure population of immature erythroblasts can be expanded to obtain 4x108 erythroblasts from 1x108 PBMC after 13-14 days in culture. Upon synchronized differentiation, high levels of enucleation (80-90\%) and low levels of cell death (",

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van den Akker, E, Satchwell, TJ, Pellegrin, S, Daniels, G & Toye, AM 2010, 'The majority of the in vitro erythroid expansion potential resides in CD34^- cells, outweighing the contribution of CD34⁺ cells and significantly increasing the erythroblast yield from peripheral blood samples', Haematologica, vol. 95, no. 9, pp. 1594 - 1598. https://doi.org/10.3324/haematol.2009.019828

The majority of the in vitro erythroid expansion potential resides in CD34^- cells, outweighing the contribution of CD34⁺ cells and significantly increasing the erythroblast yield from peripheral blood samples. / van den Akker, E; Satchwell, TJ; Pellegrin, S et al.
In: Haematologica, Vol. 95, No. 9, 01.09.2010, p. 1594 - 1598.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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