The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics

Kapil Gupta, Imre Berger

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

58 Citations (Scopus)
1725 Downloads (Pure)


Multiprotein complexes regulate most if not all cellular functions. Elucidating the structure and function of these complex cellular machines is essential for understanding biology. Moreover, multiprotein complexes by themselves constitute powerful reagents as biologics for the prevention and treatment of human diseases. Recombinant production by the baculovirus/insect cell expression system is particularly useful for expressing proteins of eukaryotic origin and their complexes. MultiBac, an advanced baculovirus/insect cell system, has been widely adopted in the last decade to produce multiprotein complexes with many subunits that were hitherto inaccessible, for academic and industrial research and development. The MultiBac system, its development and numerous applications are presented. Future opportunities for utilizing MultiBac to catalyze discovery are outlined.
Original languageEnglish
Title of host publicationAdvanced Technologies for Protein Complex Production and Characterization
EditorsM Cristina Vega
Number of pages17
ISBN (Electronic)9783319272160
ISBN (Print)9783319272146
Publication statusPublished - 2016

Publication series

NameAdvances in Experimental Medicine and Biology
PublisherSpringer International Publishing
ISSN (Print)0065-2598

Structured keywords

  • Bristol BioDesign Institute


  • Protein complexes
  • Baculovirus
  • Insect cell expression system
  • MultiBac
  • Structural biology
  • X-ray crystallography
  • Electron microscopy
  • Virus-like particles (VLPs)
  • Vaccines
  • Gene transfer
  • Lepidoptera
  • Synthetic biology


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