Bibliographical noteFunding Information:
The authors thank Dr. M. Vanevic for enabling this project with superb computational support; Dr. D. Vasishtan for providing scripts and advice that greatly facilitated subtomogram averaging; Ingr. C. T. W. M. Schneijdenberg and J. D. Meeldijk for management and maintenance of the Utrecht University EM Square facility; Stal Schep (Tull en het Waal, The Netherlands) for providing horse semen; M. W. Haaker and M. Houweling for providing mouse reproductive tracts; Prof. F. F?rster and Prof. A. Akhmanova for critical reading of the manuscript; and Prof. E. Y. Jones for insightful discussions. This work benefitted from access to the Netherlands Center for Electron Nanoscopy (NeCEN) with support from operators Dr. R. S. Dillard and Dr. C. Diebolder and IT support from B. Alewijnse. This work was funded by NWO Start-Up Grant 740.018.007 to T. Z., and M.R.L. is supported by a Clarendon Fund-Nuffield Department of Medicine Prize Studentship.
© 2021 The Authors. Published under the terms of the CC BY NC ND 4.0 license
- cryo-electron tomography
- cryo-FIB milling
- motile cilia