The production of multiprotein complexes in insect cells using the baculovirus expression system

Wassim Abdulrahman, Laura Radu, Frederic Garzoni, Olga Kolesnikova, Kapil Gupta, Judit Osz-Papai, Imre Berger, Arnaud Poterszman

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

The production of a homogeneous protein sample in sufficient quantities is an essential prerequisite not only for structural investigations but represents also a rate-limiting step for many functional studies. In the cell, a large fraction of eukaryotic proteins exists as large multicomponent assemblies with many subunits, which act in concert to catalyze specific activities. Many of these complexes cannot be obtained from endogenous source material, so recombinant expression and reconstitution are then required to overcome this bottleneck. This chapter describes current strategies and protocols for the efficient production of multiprotein complexes in large quantities and of high quality, using the baculovirus/insect cell expression system.

Original languageEnglish
Pages (from-to)91-114
Number of pages24
JournalMethods in Molecular Biology
Volume1261
DOIs
Publication statusPublished - 2015

Keywords

  • Animals
  • Baculoviridae
  • Cell Culture Techniques
  • Cell Line
  • Cloning, Molecular
  • Genetic Vectors
  • Insects
  • Multiprotein Complexes
  • Recombinant Proteins

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