The relationship between plasma angiopoietin-like protein 4 levels, angiopoietin-like protein 4 genotype, and coronary heart disease risk

Melissa C. Smart-Halajko, Marius R. Robciuc, Jackie A. Cooper, Matti Jauhiainen, Meena Kumari, Mika Kivimaki, Kay Tee Khaw, S. Matthijs Boekholdt, Nicholas J. Wareham, Tom R. Gaunt, Ian N. Day, Peter S. Braund, Christopher P. Nelson, Alistair S. Hall, Nilesh J. Samani, Steve E. Humphries, Christian Ehnholm, Philippa J. Talmud

Research output: Contribution to journalArticle (Academic Journal)peer-review

48 Citations (Scopus)

Abstract

OBJECTIVE-: To investigate the relationship between angiopoietin-like protein 4 (Angptl4) levels, coronary heart disease (CHD) biomarkers, and ANGPTL4 variants. METHODS AND RESULTS-: Plasma Angptl4 was quantified in 666 subjects of the Northwick Park Heart Study II using a validated ELISA. Seven ANGPTL4 single-nucleotide polymorphisms were genotyped, and CHD biomarkers were assessed in the whole cohort (N=2775). Weighted mean±SD plasma Angptl4 levels were 10.0±11.0 ng/mL. Plasma Angptl4 concentration correlated positively with age (r=0.15, P<0.001) and body fat mass (r=0.19, P=0.003) but negatively with plasma high-density lipoprotein cholesterol (r=-0.13, P=0.01). No correlation with triglycerides (TGs) was observed. T266M was independently associated with plasma Angptl4 levels (P<0.001) but was not associated with TGs or CHD risk in the meta-analysis of 5 studies (4061 cases/15 395 controls). E40K showed no independent association with plasma Angptl4 levels. In human embryonic kidney 293 and human hepatoma 7 cells compared with wild type, E40K and T266M showed significantly altered synthesis and secretion, respectively. CONCLUSION-: Circulating Angptl4 levels may not influence TG levels or CHD risk for the following reasons: (1) Angptl4 levels were not correlated with TGs; (2) T266M, although associated with Angptl4 levels, showed no association with plasma TGs; and (3) TG-lowering E40K did not influence Angptl4 levels. These results provide new insights into the role of Angptl4 in TG metabolism.

Original languageEnglish
Pages (from-to)2277-2282
Number of pages6
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume30
Issue number11
DOIs
Publication statusPublished - 1 Nov 2010

Keywords

  • Angptl4
  • cardiovascular disease
  • E40K
  • LPL
  • T266M

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