The retomer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network

T Wassmer, N Attar, M Harterink, J.R.T van Weering, C.J Traer, J Oakley, B Gould, D.J Stephens, P Verkade, H.C Korswagen, P.J Cullen

Research output: Contribution to journalArticle (Academic Journal)peer-review

200 Citations (Scopus)

Abstract

Early endosome-to-trans-Golgi network (TGN) transport is organized by the retromer complex. Consisting of cargo-selective and membrane-bound subcomplexes, retromer coordinates sorting with membrane deformation and carrier formation. Here, we describe four mammalian retromers whose membrane-bound subcomplexes contain specific combinations of the sorting nexins (SNX), SNX1, SNX2, SNX5, and SNX6. We establish that retromer requires a dynamic spatial organization of the endosomal network, which is regulated through association of SNX5/SNX6 with the p150glued component of dynactin, an activator of the minus-end directed microtubule motor dynein; an association further defined through genetic studies in C. elegans. Finally,wealso establish that the spatial organization of the retromer pathway is mediated through the association of SNX1 with the proposed TGN-localized tether Rab6-interacting protein-1. These interactions describe fundamental steps in retromer-mediated transport and establish that the spatial organization of the retromer network is a critical element required for efficient retromer-mediated sorting.
Translated title of the contributionThe retomer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network
Original languageEnglish
Pages (from-to)110 - 122
Number of pages13
JournalDevelopmental Cell
Volume17 (1)
Issue number1
DOIs
Publication statusPublished - Jul 2009

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