The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore

P Varanyuwatana, AP Halestrap

Research output: Contribution to journalArticle (Academic Journal)

102 Citations (Scopus)

Abstract

Phosphate activation of the mitochondrial permeability transition pore (MPTP) opening is well-documented and could involve the phosphate carrier (PiC) that we have proposed is the pore's cyclophilin-D binding component. However, others have reported that following CyP-D ablation Pi inhibits MPTP opening while cyclosporine-A (CsA) inhibits MPTP opening only when Pi is present. Here we demonstrate that Pi activates MPTP opening under all energised and de-energised conditions tested while CsA inhibits pore opening whether or not Pi is present. Using siRNA in HeLa cells we could reduce PiC expression by 65-80% but this inhibited neither mitochondrial calcium accumulation nor MPTP opening.
Translated title of the contributionThe roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore
Original languageEnglish
Pages (from-to)120 - 125
Number of pages6
JournalMitochondrion
Volume12(1)
DOIs
Publication statusPublished - Jan 2012

Bibliographical note

Other: First published on-line 8th May 2011

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