The SARS-CoV-2 Spike protein modifies the transcriptional profile of human cardiac pericytes

Elisa Avolio; Paolo R Madeddu; Prashant  Srivastava; Costanza Emanueli

The SARS-CoV-2 Spike protein modifies the transcriptional profile of human cardiac pericytes

Elisa Avolio, Paolo R Madeddu, Prashant Srivastava, Costanza Emanueli

Research output: Other contribution

Abstract

Human cardiac pericytes express the receptors for SARS-CoV-2 and contribute to microvascular dysfunction in COVID-19 patients. The SARS-CoV-2 capsid Spike protein seems to play a direct role in COVID-19 microangiopathy, but it is not known yet whether the Spike protein alone, without the infectious virus, can induce transcriptional alterations in pericytes. This study investigated the signalling pathways activated by the Spike protein in cultured human cardiac pericytes. We found that 309 RNA transcripts were significantly modulated in pericytes exposed to the Spike protein, with the upregulation of pathways linked to inflammation and viral infection.

Original language	English
Type	NCBI GEO dataset
Publication status	Published - 9 May 2023

Keywords

Cardiac pericyte
COVID-19
Spike protein
RNA-seq

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1 Article (Academic Journal)

Murine studies and expressional analyses of human cardiac pericytes reveal novel trajectories of SARS-CoV-2 Spike protein-induced microvascular damage
Avolio, E., Srivastava, P., Jiahui, J., Carrabba, M., Tsang, C. T. W., Gu, Y., Thomas, A. C., Gupta, K., Berger, I., Emanueli, C. & Madeddu, P. R., 2 Jun 2023, In: Signal Transduction and Targeted Therapy. 8, 1, 232.
Research output: Contribution to journal › Article (Academic Journal) › peer-review

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abstract = "Human cardiac pericytes express the receptors for SARS-CoV-2 and contribute to microvascular dysfunction in COVID-19 patients. The SARS-CoV-2 capsid Spike protein seems to play a direct role in COVID-19 microangiopathy, but it is not known yet whether the Spike protein alone, without the infectious virus, can induce transcriptional alterations in pericytes. This study investigated the signalling pathways activated by the Spike protein in cultured human cardiac pericytes. We found that 309 RNA transcripts were significantly modulated in pericytes exposed to the Spike protein, with the upregulation of pathways linked to inflammation and viral infection.",

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AU - Avolio, Elisa

AU - Madeddu, Paolo R

AU - Srivastava, Prashant

AU - Emanueli, Costanza

PY - 2023/5/9

Y1 - 2023/5/9

N2 - Human cardiac pericytes express the receptors for SARS-CoV-2 and contribute to microvascular dysfunction in COVID-19 patients. The SARS-CoV-2 capsid Spike protein seems to play a direct role in COVID-19 microangiopathy, but it is not known yet whether the Spike protein alone, without the infectious virus, can induce transcriptional alterations in pericytes. This study investigated the signalling pathways activated by the Spike protein in cultured human cardiac pericytes. We found that 309 RNA transcripts were significantly modulated in pericytes exposed to the Spike protein, with the upregulation of pathways linked to inflammation and viral infection.

AB - Human cardiac pericytes express the receptors for SARS-CoV-2 and contribute to microvascular dysfunction in COVID-19 patients. The SARS-CoV-2 capsid Spike protein seems to play a direct role in COVID-19 microangiopathy, but it is not known yet whether the Spike protein alone, without the infectious virus, can induce transcriptional alterations in pericytes. This study investigated the signalling pathways activated by the Spike protein in cultured human cardiac pericytes. We found that 309 RNA transcripts were significantly modulated in pericytes exposed to the Spike protein, with the upregulation of pathways linked to inflammation and viral infection.

KW - Cardiac pericyte

KW - COVID-19

KW - Spike protein

KW - RNA-seq

M3 - Other contribution

ER -

The SARS-CoV-2 Spike protein modifies the transcriptional profile of human cardiac pericytes

Abstract

Keywords

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Research output

Murine studies and expressional analyses of human cardiac pericytes reveal novel trajectories of SARS-CoV-2 Spike protein-induced microvascular damage

Cite this