The single polypeptide restriction-modification enzyme LlaGI is a self-contained molecular motor that translocates DNA loops

RM Smith, J Josephsen, MD Szczelkun

Research output: Contribution to journalArticle (Academic Journal)peer-review

12 Citations (Scopus)

Abstract

To cleave DNA, the single polypeptide restriction– modification enzyme LlaGI must communicate between a pair of indirectly repeated recognition sites. We demonstrate that this communication occurs by a 1-dimensional route, namely unidirectional dsDNA loop translocation rightward of the specific recognition sequence 50-CTnGAyG-30 as written (where n is either A, G, C or T and y is either C or T). Motion across thousands of base pairs is catalysed by the helicase domain and requires the hydrolysis of 1.5-2 ATP per base pair. DNA loop extrusion is accompanied by changes in DNA twist consistent with the motor following the helical pitch of the polynucleotide track. LlaGI is therefore an example of a polypeptide that is a completely self-contained, multi-functional molecular machine.
Translated title of the contributionThe single polypeptide restriction-modification enzyme LlaGI is a self-contained molecular motor that translocates DNA loops
Original languageEnglish
Pages (from-to)7219 - 7230
Number of pages12
JournalNucleic Acids Research
Volume37 (21)
DOIs
Publication statusPublished - Nov 2009

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