The Streptococcus gordonii adhesin CshA protein binds host fibronectin via a catch-clamp mechanism

Catherine Back, Maryta N Sztukowska, Marisa Till, Richard J Lamont, Howard F Jenkinson, Angela Nobbs*, Paul Race

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

7 Citations (Scopus)
385 Downloads (Pure)

Abstract

Adherence of bacteria to biotic or abiotic surfaces is a prerequisite for host colonization and represents an important step in microbial pathogenicity. This attachment is facilitated by bacterial adhesins at the cell surface. Because of their size and often elaborate multidomain architectures, these polypeptides represent challenging targets for detailed structural and functional characterization. The multifunctional fibrillar adhesin CshA, which mediates binding to both host molecules and other microorganisms, is an important determinant of colonization by Streptococcus gordonii, an oral commensal and opportunistic pathogen of animals and humans. CshA binds the high-molecularweight glycoprotein fibronectin (Fn) via an N-terminal non-repetitive region, and this protein-protein interaction has been proposed to promote S. gordonii colonization at multiple sites within the host. However, the molecular details of how these two proteins interact have yet to be established. Here we present a structural description of the Fn binding N-terminal region of CshA, derived from a combination of X-ray crystallography, small angle X-ray scattering, and complementary biophysical methods. In vitro binding studies support a previously unreported two-state "catch-clamp" mechanism of Fn binding by CshA, in which the disordered N-terminal domain of CshA acts to "catch" Fn, via formation of a rapidly assembled but also readily dissociable pre-complex, enabling its neighboring ligand binding domain to tightly clamp the two polypeptides together. This study presents a new paradigm for target binding by a bacterial adhesin, the identification of which will inform future efforts toward the development of anti-adhesive agents that target S. gordonii and related streptococci.

Original languageEnglish
Pages (from-to)1538-1549
Number of pages12
JournalJournal of Biological Chemistry
Volume292
Issue number5
DOIs
Publication statusPublished - 3 Feb 2017

Structured keywords

  • Bristol BioDesign Institute
  • BrisSynBio

Keywords

  • Adhesin
  • intrinsically disordered protein
  • X-ray crystallography
  • SAXS
  • bacterial pathogenesis
  • microbiology

Fingerprint Dive into the research topics of 'The Streptococcus gordonii adhesin CshA protein binds host fibronectin via a catch-clamp mechanism'. Together they form a unique fingerprint.

  • Projects

    Cite this