The toad fly Lucilia bufonivora: its evolutionary status and molecular identification

G. Arias-Robledo, Tariq Stark, R. L. Wall, J. R. Stevens*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

3 Citations (Scopus)
121 Downloads (Pure)

Abstract

The blow fly genus Lucilia is composed largely of saprophages and facultative myasis agents, including the economically important species Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae) and Lucilia sericata (Meigen). Only one species is generally recognized as an obligate agent of myiasis, Lucilia bufonivora Moniez, and this is an obligate parasite of toads. Lucilia silvarum (Meigen), a sister species, behaves mainly as a carrion breeder; however, it has also been reported as a facultative parasite of amphibians. Morphologically, these species are almost identical, and historically this has led to misidentification, taxonomic ambiguity and a paucity of studies of L. bufonivora. In this study, dipterous larvae were analysed from toad myiasis cases from the U.K., The Netherlands and Switzerland, together with adult specimens of fly species implicated in amphibian parasitism: L. bufonivora, L. silvarum and Lucilia elongata Shannon (from North America). Partial sequences of two genes, cox1 and ef1α, were amplified. Seven additional blow fly species were analysed as outgroups. Bayesian inference trees of cox1, ef1α and a combined‐gene dataset were constructed. All larvae isolated from toads were identified as L. bufonivora and no specimens of L. silvarum were implicated in amphibian myiasis. This study confirms L. silvarum and L. bufonivora as distinct sister species and provides unambiguous molecular identification of L. bufonivora.
Original languageEnglish
Pages (from-to)131-139
Number of pages9
JournalMedical and Veterinary Entomology
Volume33
Issue number1
Early online date9 Aug 2018
DOIs
Publication statusPublished - Mar 2019

Keywords

  • Animals
  • Biological Evolution
  • Bufonidae/parasitology
  • Diptera/classification
  • Electron Transport Complex IV/analysis
  • Host-Parasite Interactions
  • Insect Proteins/analysis
  • Myiasis/parasitology
  • Peptide Elongation Factor 1/analysis
  • Phylogeny
  • Sequence Analysis, DNA/veterinary

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  • Student Theses

    Lucilia blowflies: their ecology, taxonomy and the evolution of obligate amphibian parasitism

    Author: Arias Robledo, G., 1 Oct 2019

    Supervisor: Morgan, E. (Supervisor) & Wall, R. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy (PhD)

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