The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons

Bangfu Zhu; Ruth Carmichael; Luis Solabre Valois; Kevin Wilkinson; Jeremy Henley

doi:10.1016/j.bbrc.2018.04.125

The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons

Bangfu Zhu, Ruth Carmichael, Luis Solabre Valois, Kevin Wilkinson, Jeremy Henley^*

^*Corresponding author for this work

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Abstract

Neural stem cells (NSCs) are self-renewing multipotent stem cells that can be proliferated in vitro and differentiated into neuronal and/or glial lineages, making them an ideal model to study the processes involved in neuronal differentiation. Here we have used NSCs to investigate the role of the transcription factor MEF2A in neuronal differentiation and development in vitro. We show that although MEF2A is present in undifferentiated NSCs, following differentiation it is expressed at significantly higher levels in a subset of neuronal compared to non-neuronal cells. Furthermore, shRNA-mediated knockdown of MEF2A reduces the number of NSC-derived neurons compared to non-neuronal cells after differentiation. Together, these data indicate that MEF2A participates in neuronal differentiation/maturation from NSCs.

Original language	English
Pages (from-to)	645-649
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	500
Issue number	3
Early online date	23 Apr 2018
DOIs	https://doi.org/10.1016/j.bbrc.2018.04.125
Publication status	Published - 7 Jun 2018

Keywords

Development
Differentiation
MEF2
Neural stem cells
Neurons
Transcription factor

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10.1016/j.bbrc.2018.04.125

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title = "The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons",

abstract = "Neural stem cells (NSCs) are self-renewing multipotent stem cells that can be proliferated in vitro and differentiated into neuronal and/or glial lineages, making them an ideal model to study the processes involved in neuronal differentiation. Here we have used NSCs to investigate the role of the transcription factor MEF2A in neuronal differentiation and development in vitro. We show that although MEF2A is present in undifferentiated NSCs, following differentiation it is expressed at significantly higher levels in a subset of neuronal compared to non-neuronal cells. Furthermore, shRNA-mediated knockdown of MEF2A reduces the number of NSC-derived neurons compared to non-neuronal cells after differentiation. Together, these data indicate that MEF2A participates in neuronal differentiation/maturation from NSCs.",

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author = "Bangfu Zhu and Ruth Carmichael and {Solabre Valois}, Luis and Kevin Wilkinson and Jeremy Henley",

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T1 - The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons

AU - Zhu, Bangfu

AU - Carmichael, Ruth

AU - Solabre Valois, Luis

AU - Wilkinson, Kevin

AU - Henley, Jeremy

PY - 2018/6/7

Y1 - 2018/6/7

N2 - Neural stem cells (NSCs) are self-renewing multipotent stem cells that can be proliferated in vitro and differentiated into neuronal and/or glial lineages, making them an ideal model to study the processes involved in neuronal differentiation. Here we have used NSCs to investigate the role of the transcription factor MEF2A in neuronal differentiation and development in vitro. We show that although MEF2A is present in undifferentiated NSCs, following differentiation it is expressed at significantly higher levels in a subset of neuronal compared to non-neuronal cells. Furthermore, shRNA-mediated knockdown of MEF2A reduces the number of NSC-derived neurons compared to non-neuronal cells after differentiation. Together, these data indicate that MEF2A participates in neuronal differentiation/maturation from NSCs.

AB - Neural stem cells (NSCs) are self-renewing multipotent stem cells that can be proliferated in vitro and differentiated into neuronal and/or glial lineages, making them an ideal model to study the processes involved in neuronal differentiation. Here we have used NSCs to investigate the role of the transcription factor MEF2A in neuronal differentiation and development in vitro. We show that although MEF2A is present in undifferentiated NSCs, following differentiation it is expressed at significantly higher levels in a subset of neuronal compared to non-neuronal cells. Furthermore, shRNA-mediated knockdown of MEF2A reduces the number of NSC-derived neurons compared to non-neuronal cells after differentiation. Together, these data indicate that MEF2A participates in neuronal differentiation/maturation from NSCs.

KW - Development

KW - Differentiation

KW - MEF2

KW - Neural stem cells

KW - Neurons

KW - Transcription factor

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The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons

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Trafficking and Neurotrophic Effects of Botulinum Neurotoxin Type A

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The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons

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Trafficking and Neurotrophic Effects of Botulinum Neurotoxin Type A

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Wolfson Bioimaging Facility

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