The zinc finger protein cKrox directs CD4 lineage differentiation during intrathymic T cell positive selection

Guangping Sun; Xiaolong Liu; Peter Mercado; S Rhiannon Jenkinson; Magdalini Kypriotou; Lionel Feigenbaum; Philippe Galéra; Rémy Bosselut

doi:10.1038/ni1183

The zinc finger protein cKrox directs CD4 lineage differentiation during intrathymic T cell positive selection

Guangping Sun, Xiaolong Liu, Peter Mercado, S Rhiannon Jenkinson, Magdalini Kypriotou, Lionel Feigenbaum, Philippe Galéra, Rémy Bosselut

School of Cellular and Molecular Medicine

Research output: Contribution to journal › Article (Academic Journal) › peer-review

232 Citations (Scopus)

Abstract

The genetic programs directing CD4 or CD8 T cell differentiation in the thymus remain poorly understood. While analyzing gene expression during intrathymic T cell selection, we found that Zfp67, encoding the zinc finger transcription factor cKrox, was upregulated during the differentiation of CD4(+) but not CD8(+) T cells. Expression of a cKrox transgene impaired CD8 T cell development and caused major histocompatibility complex class I-restricted thymocytes to differentiate into CD4(+) T cells with helper properties rather than into cytotoxic CD8(+) T cells, as normally found. CD4 lineage differentiation mediated by cKrox required its N-terminal BTB (bric-a-brac, tramtrack, broad complex) domain. These findings identify cKrox as a chief CD4 differentiation factor during positive selection.

Original language	English
Pages (from-to)	373-81
Number of pages	9
Journal	Nature Immunology
Volume	6
Issue number	4
DOIs	https://doi.org/10.1038/ni1183
Publication status	Published - Apr 2005

Keywords

Animals
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Cell Differentiation
Cell Lineage
Humans
Immunoblotting
Mice
Mice, Inbred C57BL
Mice, Transgenic
Nerve Tissue Proteins
RNA
Receptors, Antigen, T-Cell
Repressor Proteins
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocytes, Helper-Inducer
Thymus Gland
Transcription Factors
Transcription, Genetic
Up-Regulation
Zinc Fingers

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title = "The zinc finger protein cKrox directs CD4 lineage differentiation during intrathymic T cell positive selection",

abstract = "The genetic programs directing CD4 or CD8 T cell differentiation in the thymus remain poorly understood. While analyzing gene expression during intrathymic T cell selection, we found that Zfp67, encoding the zinc finger transcription factor cKrox, was upregulated during the differentiation of CD4(+) but not CD8(+) T cells. Expression of a cKrox transgene impaired CD8 T cell development and caused major histocompatibility complex class I-restricted thymocytes to differentiate into CD4(+) T cells with helper properties rather than into cytotoxic CD8(+) T cells, as normally found. CD4 lineage differentiation mediated by cKrox required its N-terminal BTB (bric-a-brac, tramtrack, broad complex) domain. These findings identify cKrox as a chief CD4 differentiation factor during positive selection.",

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author = "Guangping Sun and Xiaolong Liu and Peter Mercado and Jenkinson, {S Rhiannon} and Magdalini Kypriotou and Lionel Feigenbaum and Philippe Gal{\'e}ra and R{\'e}my Bosselut",

year = "2005",

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doi = "10.1038/ni1183",

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T1 - The zinc finger protein cKrox directs CD4 lineage differentiation during intrathymic T cell positive selection

AU - Sun, Guangping

AU - Liu, Xiaolong

AU - Mercado, Peter

AU - Jenkinson, S Rhiannon

AU - Kypriotou, Magdalini

AU - Feigenbaum, Lionel

AU - Galéra, Philippe

AU - Bosselut, Rémy

PY - 2005/4

Y1 - 2005/4

N2 - The genetic programs directing CD4 or CD8 T cell differentiation in the thymus remain poorly understood. While analyzing gene expression during intrathymic T cell selection, we found that Zfp67, encoding the zinc finger transcription factor cKrox, was upregulated during the differentiation of CD4(+) but not CD8(+) T cells. Expression of a cKrox transgene impaired CD8 T cell development and caused major histocompatibility complex class I-restricted thymocytes to differentiate into CD4(+) T cells with helper properties rather than into cytotoxic CD8(+) T cells, as normally found. CD4 lineage differentiation mediated by cKrox required its N-terminal BTB (bric-a-brac, tramtrack, broad complex) domain. These findings identify cKrox as a chief CD4 differentiation factor during positive selection.

AB - The genetic programs directing CD4 or CD8 T cell differentiation in the thymus remain poorly understood. While analyzing gene expression during intrathymic T cell selection, we found that Zfp67, encoding the zinc finger transcription factor cKrox, was upregulated during the differentiation of CD4(+) but not CD8(+) T cells. Expression of a cKrox transgene impaired CD8 T cell development and caused major histocompatibility complex class I-restricted thymocytes to differentiate into CD4(+) T cells with helper properties rather than into cytotoxic CD8(+) T cells, as normally found. CD4 lineage differentiation mediated by cKrox required its N-terminal BTB (bric-a-brac, tramtrack, broad complex) domain. These findings identify cKrox as a chief CD4 differentiation factor during positive selection.

KW - Animals

KW - CD4-Positive T-Lymphocytes

KW - CD8-Positive T-Lymphocytes

KW - Cell Differentiation

KW - Cell Lineage

KW - Humans

KW - Immunoblotting

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - Nerve Tissue Proteins

KW - RNA

KW - Receptors, Antigen, T-Cell

KW - Repressor Proteins

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - T-Lymphocytes, Helper-Inducer

KW - Thymus Gland

KW - Transcription Factors

KW - Transcription, Genetic

KW - Up-Regulation

KW - Zinc Fingers

U2 - 10.1038/ni1183

DO - 10.1038/ni1183

M3 - Article (Academic Journal)

C2 - 15750595

SN - 1529-2908

VL - 6

SP - 373

EP - 381

JO - Nature Immunology

JF - Nature Immunology

IS - 4

ER -

The zinc finger protein cKrox directs CD4 lineage differentiation during intrathymic T cell positive selection

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Keywords

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