Three-dimensional bead position histograms reveal single-molecule nanomechanics

NB Becker, SM Altmann, T Scholz, JKH Hoerber, EHK Stelzer, A Rohrbach

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

We describe a method to investigate the structure and elasticity of macromolecules by a combination of single molecule experiments and kinematic modeling. With a photonic force microscope, we recorded spatial position histograms of a fluctuating microsphere tethered to full-length myosin-II. Assuming only that the molecule consists of concatenated rigid segments, a model derived from robot kinematics allows us to relate these histograms to the molecule's segment lengths and bending stiffnesses. Both our calculated position distributions and the experimental data show an asymmetry characteristic of a mixed entropic-enthalpic spring. Our model that fits best to experimental line profiles has two intramolecular hinges, one at the bound head domain, and another about 50 nm down the myosin tail, with a summed bending stiffness of about 3 k(B)T/rad.
Translated title of the contributionThree-dimensional bead position histograms reveal single-molecule nanomechanics
Original languageEnglish
Article numberPart 1
Pages (from-to)021907
JournalPhysical Review E: Statistical, Nonlinear, and Soft Matter Physics
Volume71(2)
Publication statusPublished - 2005

Bibliographical note

Publisher: American Physical Society

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