Abstract
PURPOSE. We investigated single nucleotide polymorphisms (SNPs) of thrombospondin-1 (TSP-1) on the risk of corneal allograft rejection. The TSP-1 is known to be involved in the immune response of the anterior chamber of the eye, activating TGF-b2, promoting peripheral and systemic tolerance, and counteracting the proangiogenic activity of VEGF.
METHODS. Three tagging SNPs spanning the TSP-1 region (rs1478604, A>G; rs2228261, C>T; and rs2228262, A>G) were genotyped. Association with risk of rejection was investigated in a group of 378 corneal transplant recipients with risk factors for allograft rejection. Transplant recipients had completed 3-year follow-up.
RESULTS. The TSP-1 rs1478604 A SNP was associated significantly with an increased risk of corneal allograft rejection (odds ratio [OR], 1.58; 95% confidence interval [CI], 1.02–2.45; P= 0.04) and there was a trend toward the rs1478604, rs2228261, rs2228262 ACA haplotypeincreasing risk of rejection.
CONCLUSIONS. The results suggest that TSP-1 rs1478604 AA homozygotes may be at increased risk of corneal transplant rejection, especially if they carry the ACA haplotype.
Keywords: corneal transplantation, thrombospondin-1, single nucleotide polymorphism
METHODS. Three tagging SNPs spanning the TSP-1 region (rs1478604, A>G; rs2228261, C>T; and rs2228262, A>G) were genotyped. Association with risk of rejection was investigated in a group of 378 corneal transplant recipients with risk factors for allograft rejection. Transplant recipients had completed 3-year follow-up.
RESULTS. The TSP-1 rs1478604 A SNP was associated significantly with an increased risk of corneal allograft rejection (odds ratio [OR], 1.58; 95% confidence interval [CI], 1.02–2.45; P= 0.04) and there was a trend toward the rs1478604, rs2228261, rs2228262 ACA haplotypeincreasing risk of rejection.
CONCLUSIONS. The results suggest that TSP-1 rs1478604 AA homozygotes may be at increased risk of corneal transplant rejection, especially if they carry the ACA haplotype.
Keywords: corneal transplantation, thrombospondin-1, single nucleotide polymorphism
| Original language | English |
|---|---|
| Article number | DOI: 10.1167/iovs.13-13681 |
| Pages (from-to) | 2115-2120 |
| Number of pages | 5 |
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 55 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 7 Apr 2014 |
Keywords
- corneal transplantation
- thrombospondin-1
- Single nucleotide polymorphism