Tracing the rate and extent of N and C flow from C-13,N-15-glycine and glutamate into individual de novo synthesised soil amino acids

Timothy D. J. Knowles; David R. Chadwick; Roland Bol; Richard P. Evershed

doi:10.1016/j.orggeochem.2010.09.003

Tracing the rate and extent of N and C flow from C-13,N-15-glycine and glutamate into individual de novo synthesised soil amino acids

Timothy D. J. Knowles, David R. Chadwick, Roland Bol, Richard P. Evershed^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

41 Citations (Scopus)

Abstract

Mineralisation rates provide valuable information concerning the overall cycling of soil organic N; however, detailed information regarding the pathways preceding the mineralisation of organic substrates remains elusive. We have adopted a molecular approach to open the 'black box' of organic N cycling in soil. Stable isotope probing employing compound-specific isotopic analysis was used to trace the fate of N and C within metabolites central to organic N cycling. In time course experiments, N-15 and C-13 from two dual-labelled amino acid (AA) substrates (U-C-13,N-15-glutamate and U-C-13,N-15-glycine) were followed into AAs biosynthesised de novo. In the majority of cases, highly significant differences (P

Original language	English
Pages (from-to)	1259-1268
Number of pages	10
Journal	Organic Geochemistry
Volume	41
Issue number	12
DOIs	https://doi.org/10.1016/j.orggeochem.2010.09.003
Publication status	Published - Dec 2010

Keywords

ORGANIC NITROGEN
ESCHERICHIA-COLI
MASS-SPECTROMETRY
DELTA-C-13 VALUES
SERINE HYDROXYMETHYLTRANSFERASE
GLYCINE-CLEAVAGE
CARBON
MINERALIZATION
METABOLISM
TURNOVER

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title = "Tracing the rate and extent of N and C flow from C-13,N-15-glycine and glutamate into individual de novo synthesised soil amino acids",

abstract = "Mineralisation rates provide valuable information concerning the overall cycling of soil organic N; however, detailed information regarding the pathways preceding the mineralisation of organic substrates remains elusive. We have adopted a molecular approach to open the 'black box' of organic N cycling in soil. Stable isotope probing employing compound-specific isotopic analysis was used to trace the fate of N and C within metabolites central to organic N cycling. In time course experiments, N-15 and C-13 from two dual-labelled amino acid (AA) substrates (U-C-13,N-15-glutamate and U-C-13,N-15-glycine) were followed into AAs biosynthesised de novo. In the majority of cases, highly significant differences (P",

keywords = "ORGANIC NITROGEN, ESCHERICHIA-COLI, MASS-SPECTROMETRY, DELTA-C-13 VALUES, SERINE HYDROXYMETHYLTRANSFERASE, GLYCINE-CLEAVAGE, CARBON, MINERALIZATION, METABOLISM, TURNOVER",

author = "Knowles, {Timothy D. J.} and Chadwick, {David R.} and Roland Bol and Evershed, {Richard P.}",

year = "2010",

month = dec,

doi = "10.1016/j.orggeochem.2010.09.003",

language = "English",

volume = "41",

pages = "1259--1268",

journal = "Organic Geochemistry",

issn = "0146-6380",

publisher = "Elsevier",

number = "12",

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TY - JOUR

T1 - Tracing the rate and extent of N and C flow from C-13,N-15-glycine and glutamate into individual de novo synthesised soil amino acids

AU - Knowles, Timothy D. J.

AU - Chadwick, David R.

AU - Bol, Roland

AU - Evershed, Richard P.

PY - 2010/12

Y1 - 2010/12

N2 - Mineralisation rates provide valuable information concerning the overall cycling of soil organic N; however, detailed information regarding the pathways preceding the mineralisation of organic substrates remains elusive. We have adopted a molecular approach to open the 'black box' of organic N cycling in soil. Stable isotope probing employing compound-specific isotopic analysis was used to trace the fate of N and C within metabolites central to organic N cycling. In time course experiments, N-15 and C-13 from two dual-labelled amino acid (AA) substrates (U-C-13,N-15-glutamate and U-C-13,N-15-glycine) were followed into AAs biosynthesised de novo. In the majority of cases, highly significant differences (P

AB - Mineralisation rates provide valuable information concerning the overall cycling of soil organic N; however, detailed information regarding the pathways preceding the mineralisation of organic substrates remains elusive. We have adopted a molecular approach to open the 'black box' of organic N cycling in soil. Stable isotope probing employing compound-specific isotopic analysis was used to trace the fate of N and C within metabolites central to organic N cycling. In time course experiments, N-15 and C-13 from two dual-labelled amino acid (AA) substrates (U-C-13,N-15-glutamate and U-C-13,N-15-glycine) were followed into AAs biosynthesised de novo. In the majority of cases, highly significant differences (P

KW - ORGANIC NITROGEN

KW - ESCHERICHIA-COLI

KW - MASS-SPECTROMETRY

KW - DELTA-C-13 VALUES

KW - SERINE HYDROXYMETHYLTRANSFERASE

KW - GLYCINE-CLEAVAGE

KW - CARBON

KW - MINERALIZATION

KW - METABOLISM

KW - TURNOVER

U2 - 10.1016/j.orggeochem.2010.09.003

DO - 10.1016/j.orggeochem.2010.09.003

M3 - Article (Academic Journal)

SN - 0146-6380

VL - 41

SP - 1259

EP - 1268

JO - Organic Geochemistry

JF - Organic Geochemistry

IS - 12

ER -

Tracing the rate and extent of N and C flow from C-13,N-15-glycine and glutamate into individual de novo synthesised soil amino acids

Abstract

Keywords

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