Tracing the rate and extent of N and C flow from C-13,N-15-glycine and glutamate into individual de novo synthesised soil amino acids

Timothy D. J. Knowles, David R. Chadwick, Roland Bol, Richard P. Evershed*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

24 Citations (Scopus)

Abstract

Mineralisation rates provide valuable information concerning the overall cycling of soil organic N; however, detailed information regarding the pathways preceding the mineralisation of organic substrates remains elusive. We have adopted a molecular approach to open the 'black box' of organic N cycling in soil. Stable isotope probing employing compound-specific isotopic analysis was used to trace the fate of N and C within metabolites central to organic N cycling. In time course experiments, N-15 and C-13 from two dual-labelled amino acid (AA) substrates (U-C-13,N-15-glutamate and U-C-13,N-15-glycine) were followed into AAs biosynthesised de novo. In the majority of cases, highly significant differences (P

Original languageEnglish
Pages (from-to)1259-1268
Number of pages10
JournalOrganic Geochemistry
Volume41
Issue number12
DOIs
Publication statusPublished - Dec 2010

Keywords

  • ORGANIC NITROGEN
  • ESCHERICHIA-COLI
  • MASS-SPECTROMETRY
  • DELTA-C-13 VALUES
  • SERINE HYDROXYMETHYLTRANSFERASE
  • GLYCINE-CLEAVAGE
  • CARBON
  • MINERALIZATION
  • METABOLISM
  • TURNOVER

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