Tracking Cardiovascular Comorbidity in Models of Chronic Inflammatory Disease

Aisling S Morrin; Simon Eastham; Anwen S Williams; Gareth W Jones

doi:10.1007/978-1-0716-3331-1_10

Tracking Cardiovascular Comorbidity in Models of Chronic Inflammatory Disease

Aisling S Morrin, Simon Eastham, Anwen S Williams, Gareth W Jones

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

Abstract

Immune-mediated inflammatory diseases (IMIDs) are commonly associated with complex coexisting conditions, and cardiovascular comorbidities are a common cause of mortality in systemic inflammation. Experimental models of disease provide an opportunity to dissect inflammatory mechanisms that promote damage to vascular tissues affected by comorbidity. Here, we describe methods to recover the thoracic aorta from mice during experimental inflammatory arthritis and assess vascular constriction responses by isometric tension myography. To complement the assessment of functional changes in the vasculature during inflammatory arthritis, we also outline a method to characterize vascular inflammation by immunohistochemistry.

Original language	English
Title of host publication	Inflammation and Cancer
Subtitle of host publication	Methods and Protocols
Editors	Brendan J. Jenkins
Publisher	Springer
Pages	123-137
Number of pages	15
Volume	2691
ISBN (Electronic)	978-1-0716-3331-1
ISBN (Print)	978-1-0716-3330-4
DOIs	https://doi.org/10.1007/978-1-0716-3331-1_10
Publication status	Published - 25 Jun 2023

Publication series

Name	Methods in Molecular Biology
Volume	2691
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Funding Information:
This work was supported by funding from Versus Arthritis (refer-ence 22706). SE is supported by a Fight for Sight and Versus Arthritis jointly funded PhD scholarship (reference 5149 / 5150) and ASM by an industry sponsored PhD studentship from Galecto Biotech AB.

Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Animals
Mice
Comorbidity
Inflammation/complications
Aorta, Thoracic
Arthritis, Experimental/complications
Cardiovascular Diseases/etiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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abstract = "Immune-mediated inflammatory diseases (IMIDs) are commonly associated with complex coexisting conditions, and cardiovascular comorbidities are a common cause of mortality in systemic inflammation. Experimental models of disease provide an opportunity to dissect inflammatory mechanisms that promote damage to vascular tissues affected by comorbidity. Here, we describe methods to recover the thoracic aorta from mice during experimental inflammatory arthritis and assess vascular constriction responses by isometric tension myography. To complement the assessment of functional changes in the vasculature during inflammatory arthritis, we also outline a method to characterize vascular inflammation by immunohistochemistry.",

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note = "Funding Information: This work was supported by funding from Versus Arthritis (refer-ence 22706). SE is supported by a Fight for Sight and Versus Arthritis jointly funded PhD scholarship (reference 5149 / 5150) and ASM by an industry sponsored PhD studentship from Galecto Biotech AB. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

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Tracking Cardiovascular Comorbidity in Models of Chronic Inflammatory Disease. / Morrin, Aisling S; Eastham, Simon; Williams, Anwen S et al.
Inflammation and Cancer: Methods and Protocols. ed. / Brendan J. Jenkins. Vol. 2691 Springer, 2023. p. 123-137 (Methods in Molecular Biology; Vol. 2691).

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

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PY - 2023/6/25

Y1 - 2023/6/25

N2 - Immune-mediated inflammatory diseases (IMIDs) are commonly associated with complex coexisting conditions, and cardiovascular comorbidities are a common cause of mortality in systemic inflammation. Experimental models of disease provide an opportunity to dissect inflammatory mechanisms that promote damage to vascular tissues affected by comorbidity. Here, we describe methods to recover the thoracic aorta from mice during experimental inflammatory arthritis and assess vascular constriction responses by isometric tension myography. To complement the assessment of functional changes in the vasculature during inflammatory arthritis, we also outline a method to characterize vascular inflammation by immunohistochemistry.

AB - Immune-mediated inflammatory diseases (IMIDs) are commonly associated with complex coexisting conditions, and cardiovascular comorbidities are a common cause of mortality in systemic inflammation. Experimental models of disease provide an opportunity to dissect inflammatory mechanisms that promote damage to vascular tissues affected by comorbidity. Here, we describe methods to recover the thoracic aorta from mice during experimental inflammatory arthritis and assess vascular constriction responses by isometric tension myography. To complement the assessment of functional changes in the vasculature during inflammatory arthritis, we also outline a method to characterize vascular inflammation by immunohistochemistry.

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KW - Mice

KW - Comorbidity

KW - Inflammation/complications

KW - Aorta, Thoracic

KW - Arthritis, Experimental/complications

KW - Cardiovascular Diseases/etiology

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PB - Springer

ER -

Tracking Cardiovascular Comorbidity in Models of Chronic Inflammatory Disease

Abstract

Publication series

Bibliographical note

Keywords

UN SDGs

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