Tracking Cardiovascular Comorbidity in Models of Chronic Inflammatory Disease

Aisling S Morrin, Simon Eastham, Anwen S Williams, Gareth W Jones

Research output: Chapter in Book/Report/Conference proceedingChapter in a book


Immune-mediated inflammatory diseases (IMIDs) are commonly associated with complex coexisting conditions, and cardiovascular comorbidities are a common cause of mortality in systemic inflammation. Experimental models of disease provide an opportunity to dissect inflammatory mechanisms that promote damage to vascular tissues affected by comorbidity. Here, we describe methods to recover the thoracic aorta from mice during experimental inflammatory arthritis and assess vascular constriction responses by isometric tension myography. To complement the assessment of functional changes in the vasculature during inflammatory arthritis, we also outline a method to characterize vascular inflammation by immunohistochemistry.

Original languageEnglish
Title of host publicationInflammation and Cancer
Subtitle of host publicationMethods and Protocols
EditorsBrendan J. Jenkins
Number of pages15
ISBN (Electronic)978-1-0716-3331-1
ISBN (Print)978-1-0716-3330-4
Publication statusPublished - 25 Jun 2023

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Funding Information:
This work was supported by funding from Versus Arthritis (refer-ence 22706). SE is supported by a Fight for Sight and Versus Arthritis jointly funded PhD scholarship (reference 5149 / 5150) and ASM by an industry sponsored PhD studentship from Galecto Biotech AB.

Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


  • Animals
  • Mice
  • Comorbidity
  • Inflammation/complications
  • Aorta, Thoracic
  • Arthritis, Experimental/complications
  • Cardiovascular Diseases/etiology


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