TY - JOUR
T1 - Transcriptome analysis of Streptococcus gordonii Challis DL1 indicates a role for the biofilm-associated fruRBA operon in response to Candida albicans
AU - Jesionowski, A M
AU - Mansfield, J M
AU - Brittan, Jane L
AU - Jenkinson, Howard F
AU - Vickerman, M Margaret
N1 - © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
PY - 2016/8
Y1 - 2016/8
N2 - Multiple levels of interkingdom signaling have been implicated in maintaining the ecological balance between Candida albicans
and commensal streptococci to assure a state of oral health. To better
understand the molecular mechanisms involved in the initial
streptococcal response to the presence of C. albicans that can initiate oral surface colonization and biofilm formation, hypha-forming cells were incubated with Streptococcus gordonii
cells for 30 min to assess the streptococcal transcriptome response. A
genome-wide microarray analysis and quantitative polymerase chain
reaction validation of S. gordonii transcripts identified a
number of genes, the majority of which were involved in metabolic
functions that were differentially expressed in the presence of hyphae.
The fruR, fruB, and fruA genes encoding the
transcriptional regulator, fructose-1-phosphate kinase, and
fructose-specific permease, respectively, of the
phosphoenolpyruvate-dependent fructose phosphotransferase system, were
consistently upregulated. An S. gordonii mutant in which these genes were deleted by allelic replacement formed an architecturally distinct, less robust biofilm with C. albicans than did parental strain cells. Complementing the mutant with plasmid borne fruR, fruB, and fruA
genes caused phenotype reversion, indicating that the genes in this
operon played a role in dual-species biofilm formation. This genome-wide
analysis of the S. gordonii transcriptional response to C. albicans has identified several genes that have potential roles in interkingdom signaling and responses.
AB - Multiple levels of interkingdom signaling have been implicated in maintaining the ecological balance between Candida albicans
and commensal streptococci to assure a state of oral health. To better
understand the molecular mechanisms involved in the initial
streptococcal response to the presence of C. albicans that can initiate oral surface colonization and biofilm formation, hypha-forming cells were incubated with Streptococcus gordonii
cells for 30 min to assess the streptococcal transcriptome response. A
genome-wide microarray analysis and quantitative polymerase chain
reaction validation of S. gordonii transcripts identified a
number of genes, the majority of which were involved in metabolic
functions that were differentially expressed in the presence of hyphae.
The fruR, fruB, and fruA genes encoding the
transcriptional regulator, fructose-1-phosphate kinase, and
fructose-specific permease, respectively, of the
phosphoenolpyruvate-dependent fructose phosphotransferase system, were
consistently upregulated. An S. gordonii mutant in which these genes were deleted by allelic replacement formed an architecturally distinct, less robust biofilm with C. albicans than did parental strain cells. Complementing the mutant with plasmid borne fruR, fruB, and fruA
genes caused phenotype reversion, indicating that the genes in this
operon played a role in dual-species biofilm formation. This genome-wide
analysis of the S. gordonii transcriptional response to C. albicans has identified several genes that have potential roles in interkingdom signaling and responses.
KW - coaggregation
KW - interkingdom signaling
KW - messenger RNA
KW - phosphotransferase system
KW - polymicrobial communities
U2 - 10.1111/omi.12125
DO - 10.1111/omi.12125
M3 - Article (Academic Journal)
C2 - 26280461
SN - 2041-1006
VL - 31
SP - 314
EP - 328
JO - Molecular Oral Microbiology
JF - Molecular Oral Microbiology
IS - 4
ER -