Transcriptome analysis of Streptococcus gordonii Challis DL1 indicates a role for the biofilm-associated fruRBA operon in response to Candida albicans

A M Jesionowski, J M Mansfield, Jane L Brittan, Howard F Jenkinson, M Margaret Vickerman

Research output: Contribution to journalArticle (Academic Journal)

2 Citations (Scopus)
211 Downloads (Pure)

Abstract

Multiple levels of interkingdom signaling have been implicated in maintaining the ecological balance between Candida albicans and commensal streptococci to assure a state of oral health. To better understand the molecular mechanisms involved in the initial streptococcal response to the presence of C. albicans that can initiate oral surface colonization and biofilm formation, hypha-forming cells were incubated with Streptococcus gordonii cells for 30 min to assess the streptococcal transcriptome response. A genome-wide microarray analysis and quantitative polymerase chain reaction validation of S. gordonii transcripts identified a number of genes, the majority of which were involved in metabolic functions that were differentially expressed in the presence of hyphae. The fruR, fruB, and fruA genes encoding the transcriptional regulator, fructose-1-phosphate kinase, and fructose-specific permease, respectively, of the phosphoenolpyruvate-dependent fructose phosphotransferase system, were consistently upregulated. An S. gordonii mutant in which these genes were deleted by allelic replacement formed an architecturally distinct, less robust biofilm with C. albicans than did parental strain cells. Complementing the mutant with plasmid borne fruR, fruB, and fruA genes caused phenotype reversion, indicating that the genes in this operon played a role in dual-species biofilm formation. This genome-wide analysis of the S. gordonii transcriptional response to C. albicans has identified several genes that have potential roles in interkingdom signaling and responses.
Original languageEnglish
Pages (from-to)314-328
Number of pages15
JournalMolecular Oral Microbiology
Volume31
Issue number4
Early online date25 Sep 2015
DOIs
Publication statusPublished - Aug 2016

Keywords

  • coaggregation
  • interkingdom signaling
  • messenger RNA
  • phosphotransferase system
  • polymicrobial communities

Fingerprint Dive into the research topics of 'Transcriptome analysis of <i>Streptococcus gordonii </i>Challis DL1 indicates a role for the biofilm-associated <i>fruRBA </i>operon in response to <i>Candida albicans</i>'. Together they form a unique fingerprint.

Cite this