Transgenesis has less impact on the transcriptome wheat grain than conventional breeding

MM Baudo, R Lyons, S Powers, GM Pastori, KJ Edwards, MJ Holdsworth, PR Shewry

Research output: Contribution to journalArticle (Academic Journal)peer-review

122 Citations (Scopus)

Abstract

Detailed global gene expression profiles have been obtained for a series of transgenic and conventionally bred wheat lines expressing additional genes encoding HMW (high molecular weight) subunits of glutenin, a group of endosperm-specific seed storage proteins known to determine dough strength and therefore bread-making quality. Differences in endosperm and leaf transcriptome profiles between untransformed and derived transgenic lines were consistently extremely small, when analysing plants containing either transgenes only, or also marker genes. Differences observed in gene expression in the endosperm between conventionally bred material were much larger in comparison to differences between transgenic and untransformed lines exhibiting the same complements of gluten subunits. These results suggest that the presence of the transgenes did not significantly alter gene expression and that, at this level of investigation, transgenic plants could be considered substantially equivalent to untransformed parental lines.
Translated title of the contributionTransgenesis has less impact on the transcriptome wheat grain than conventional breeding
Original languageEnglish
Pages (from-to)369 - 380
Number of pages12
JournalPlant Biotechnology Journal
Volume4 (4)
DOIs
Publication statusPublished - Jul 2006

Bibliographical note

Publisher: Blackwell

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