Transplatation of low dose CD34+KDR+ cells promotes vcascular and muscular regeneration in ischemic limbs

PR Madeddu, C Emanueli, E Pelosi, MB Salis, AM Cerio, G Bonanno, M Patti, G Stassi, G Condorelli, C Peschle

Research output: Contribution to journalArticle (Academic Journal)peer-review

109 Citations (Scopus)

Abstract

Hematopoietic progenitor cell transplantation can contribute to revascularization of ischemic tissues. Yet, the optimal cell population to be transplanted has yet to be determined. We have compared the therapeutic potential of two subsets of human cord blood CD34+ progenitors, either expressing the VEGF-A receptor 2 (KDR) or not. In serum-free starvation culture, CD34+KDR+ cells reportedly showed greater resistance to apoptosis and ability to release VEGF-A, as compared with CD34+KDR- cells. When injected into the hind muscles in immunodeficient SCIDbg mice subjected to unilateral ischemia, a low number (10(3)) of CD34+KDR+ cells improved limb salvage and hemodynamic recovery better than a larger dosage (10(4)) of CD34+KDR- cells. The neovascularization induced by KDR+ cells was significantly superior to that promoted by KDR- cells. Similarly, endothelial cell apoptosis and interstitial fibrosis were significantly attenuated by KDR+ cells, which differentiated into mature human endothelial cells and also apparently skeletal muscle cells. This study demonstrates that a low number of CD34+KDR+ cells favors reparative neovascularization and possibly myogenesis in limb ischemia, suggesting the potential use of this cell population in regenerative medicine.

Translated title of the contributionTransplatation of low dose CD34+KDR+ cells promotes vcascular and muscular regeneration in ischemic limbs
Original languageEnglish
Pages (from-to)1737 - 1739
Number of pages3
JournalFASEB Journal
Volume18
Issue number14
DOIs
Publication statusPublished - Nov 2004

Bibliographical note

Publisher: Federation of American Societies for Exp. Biology

Keywords

  • Animals
  • Antigens, CD34
  • Apoptosis
  • Extremities
  • Fetal Blood
  • Fibrosis
  • Hemodynamics
  • Humans
  • Ischemia
  • Mice
  • Muscle Fibers, Skeletal
  • Muscle, Skeletal
  • Neovascularization, Physiologic
  • Regeneration
  • Stem Cell Transplantation
  • Stem Cells
  • Vascular Endothelial Growth Factor Receptor-2

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