Triglyceride content increases while cholesterol content decreases in HDL and LDL+IDL fractions following normal meals: The Copenhagen General Population Study of 25,656 individuals

Mia Ø Johansen; Juan Moreno-Vedia; Mie Balling; George Davey Smith; Børge G Nordestgaard

doi:10.1016/j.atherosclerosis.2023.117316

Triglyceride content increases while cholesterol content decreases in HDL and LDL+IDL fractions following normal meals: The Copenhagen General Population Study of 25,656 individuals

Mia Ø Johansen, Juan Moreno-Vedia, Mie Balling, George Davey Smith, Børge G Nordestgaard^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

4 Citations (Scopus)

Abstract

BACKGROUND AND AIMS: During fat tolerance tests, plasma triglycerides increase while high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and intermediate-density lipoprotein (IDL) cholesterol decrease. However, it is unknown whether triglyceride content increases and cholesterol content decreases in HDL and LDL + IDL fractions following normal meals in the general population. Therefore, we tested the hypothesis that triglyceride content increases while cholesterol content decreases in HDL and LDL + IDL fractions following normal meals.

METHODS: In this cross-sectional study, we included 25,656 individuals aged 20-100 years, all without lipid-lowering therapy at examination and selected for metabolomic profiling from the Copenhagen General Population Study. Triglyceride and cholesterol content of 14 lipoprotein fractions weas measured using nuclear magnetic resonance (NMR) spectroscopy. Time since last meal was recorded by the examiner immediately before blood sampling.

RESULTS: Following normal meals in age and sex-adjusted analyses and when compared with fasting levels, plasma triglycerides were higher for up to 5-6 h, and triglyceride content was higher for up to 6-7 h in HDL fractions, for up to 6-7 h in LDL + IDL fractions, and for up to 5-6 h in very-low-density lipoprotein (VLDL) fractions. Further, plasma cholesterol was lower for up to 2-3 h, and cholesterol content was lower for up to 0-1 h in HDL fractions and for up to 4-5 h in LDL + IDL fractions, while cholesterol content was higher for up to 4-5 h in VLDL fractions.

CONCLUSIONS: Following normal meals, triglyceride content increases while cholesterol content decreases in HDL and LDL + IDL fractions.

Original language	English
Article number	117316
Journal	Atherosclerosis
Volume	383
Early online date	27 Sept 2023
DOIs	https://doi.org/10.1016/j.atherosclerosis.2023.117316
Publication status	Published - 10 Oct 2023

Bibliographical note

Funding Information:
The study was funded by the Independent Research Fund, Denmark [grantno: 9039-00360B to BGN], and by Overlæge Johan Boserup and Lise Boserups Grant [grant no: 20795–24 to MØJ]. George Davey Smith and NMR processing costs were supported by the Medical Research Council Integrative Epidemiology Unit at the University of Bristol MC_UU_00011/1. Funders had no role in study design, data collection, data analysis, data interpretation, writing of the report, or in the decision to submit the paper for publication.

Publisher Copyright:
© 2023 The Authors

Research Groups and Themes

Bristol Population Health Science Institute

Keywords

Humans
Triglycerides
Cross-Sectional Studies
Cholesterol
Lipoproteins, LDL
Lipoproteins, VLDL
Cholesterol, HDL

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10.1016/j.atherosclerosis.2023.117316Licence: CC BY

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IEU: MRC Integrative Epidemiology Unit Quinquennial renewal
Gaunt, L. F. (Principal Investigator) & Davey Smith, G. (Principal Investigator)
1/04/18 → 31/03/23
Project: Research

Cite this

Johansen, M. Ø., Moreno-Vedia, J., Balling, M., Davey Smith, G., & Nordestgaard, B. G. (2023). Triglyceride content increases while cholesterol content decreases in HDL and LDL+IDL fractions following normal meals: The Copenhagen General Population Study of 25,656 individuals. Atherosclerosis, 383, Article 117316. https://doi.org/10.1016/j.atherosclerosis.2023.117316

@article{e6e75e2a1f004482816c4b6bb4b2e014,

title = "Triglyceride content increases while cholesterol content decreases in HDL and LDL+IDL fractions following normal meals: The Copenhagen General Population Study of 25,656 individuals",

abstract = "BACKGROUND AND AIMS: During fat tolerance tests, plasma triglycerides increase while high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and intermediate-density lipoprotein (IDL) cholesterol decrease. However, it is unknown whether triglyceride content increases and cholesterol content decreases in HDL and LDL + IDL fractions following normal meals in the general population. Therefore, we tested the hypothesis that triglyceride content increases while cholesterol content decreases in HDL and LDL + IDL fractions following normal meals.METHODS: In this cross-sectional study, we included 25,656 individuals aged 20-100 years, all without lipid-lowering therapy at examination and selected for metabolomic profiling from the Copenhagen General Population Study. Triglyceride and cholesterol content of 14 lipoprotein fractions weas measured using nuclear magnetic resonance (NMR) spectroscopy. Time since last meal was recorded by the examiner immediately before blood sampling.RESULTS: Following normal meals in age and sex-adjusted analyses and when compared with fasting levels, plasma triglycerides were higher for up to 5-6 h, and triglyceride content was higher for up to 6-7 h in HDL fractions, for up to 6-7 h in LDL + IDL fractions, and for up to 5-6 h in very-low-density lipoprotein (VLDL) fractions. Further, plasma cholesterol was lower for up to 2-3 h, and cholesterol content was lower for up to 0-1 h in HDL fractions and for up to 4-5 h in LDL + IDL fractions, while cholesterol content was higher for up to 4-5 h in VLDL fractions.CONCLUSIONS: Following normal meals, triglyceride content increases while cholesterol content decreases in HDL and LDL + IDL fractions.",

keywords = "Humans, Triglycerides, Cross-Sectional Studies, Cholesterol, Lipoproteins, LDL, Lipoproteins, VLDL, Cholesterol, HDL",

author = "Johansen, {Mia {\O}} and Juan Moreno-Vedia and Mie Balling and {Davey Smith}, George and Nordestgaard, {B{\o}rge G}",

note = "Funding Information: The study was funded by the Independent Research Fund, Denmark [grantno: 9039-00360B to BGN], and by Overl{\ae}ge Johan Boserup and Lise Boserups Grant [grant no: 20795–24 to M{\O}J]. George Davey Smith and NMR processing costs were supported by the Medical Research Council Integrative Epidemiology Unit at the University of Bristol MC_UU_00011/1. Funders had no role in study design, data collection, data analysis, data interpretation, writing of the report, or in the decision to submit the paper for publication. Publisher Copyright: {\textcopyright} 2023 The Authors",

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doi = "10.1016/j.atherosclerosis.2023.117316",

language = "English",

volume = "383",

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Triglyceride content increases while cholesterol content decreases in HDL and LDL+IDL fractions following normal meals: The Copenhagen General Population Study of 25,656 individuals. / Johansen, Mia Ø; Moreno-Vedia, Juan; Balling, Mie et al.
In: Atherosclerosis, Vol. 383, 117316, 10.10.2023.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

TY - JOUR

T1 - Triglyceride content increases while cholesterol content decreases in HDL and LDL+IDL fractions following normal meals

T2 - The Copenhagen General Population Study of 25,656 individuals

AU - Johansen, Mia Ø

AU - Moreno-Vedia, Juan

AU - Balling, Mie

AU - Davey Smith, George

AU - Nordestgaard, Børge G

N1 - Funding Information: The study was funded by the Independent Research Fund, Denmark [grantno: 9039-00360B to BGN], and by Overlæge Johan Boserup and Lise Boserups Grant [grant no: 20795–24 to MØJ]. George Davey Smith and NMR processing costs were supported by the Medical Research Council Integrative Epidemiology Unit at the University of Bristol MC_UU_00011/1. Funders had no role in study design, data collection, data analysis, data interpretation, writing of the report, or in the decision to submit the paper for publication. Publisher Copyright: © 2023 The Authors

PY - 2023/10/10

Y1 - 2023/10/10

N2 - BACKGROUND AND AIMS: During fat tolerance tests, plasma triglycerides increase while high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and intermediate-density lipoprotein (IDL) cholesterol decrease. However, it is unknown whether triglyceride content increases and cholesterol content decreases in HDL and LDL + IDL fractions following normal meals in the general population. Therefore, we tested the hypothesis that triglyceride content increases while cholesterol content decreases in HDL and LDL + IDL fractions following normal meals.METHODS: In this cross-sectional study, we included 25,656 individuals aged 20-100 years, all without lipid-lowering therapy at examination and selected for metabolomic profiling from the Copenhagen General Population Study. Triglyceride and cholesterol content of 14 lipoprotein fractions weas measured using nuclear magnetic resonance (NMR) spectroscopy. Time since last meal was recorded by the examiner immediately before blood sampling.RESULTS: Following normal meals in age and sex-adjusted analyses and when compared with fasting levels, plasma triglycerides were higher for up to 5-6 h, and triglyceride content was higher for up to 6-7 h in HDL fractions, for up to 6-7 h in LDL + IDL fractions, and for up to 5-6 h in very-low-density lipoprotein (VLDL) fractions. Further, plasma cholesterol was lower for up to 2-3 h, and cholesterol content was lower for up to 0-1 h in HDL fractions and for up to 4-5 h in LDL + IDL fractions, while cholesterol content was higher for up to 4-5 h in VLDL fractions.CONCLUSIONS: Following normal meals, triglyceride content increases while cholesterol content decreases in HDL and LDL + IDL fractions.

AB - BACKGROUND AND AIMS: During fat tolerance tests, plasma triglycerides increase while high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and intermediate-density lipoprotein (IDL) cholesterol decrease. However, it is unknown whether triglyceride content increases and cholesterol content decreases in HDL and LDL + IDL fractions following normal meals in the general population. Therefore, we tested the hypothesis that triglyceride content increases while cholesterol content decreases in HDL and LDL + IDL fractions following normal meals.METHODS: In this cross-sectional study, we included 25,656 individuals aged 20-100 years, all without lipid-lowering therapy at examination and selected for metabolomic profiling from the Copenhagen General Population Study. Triglyceride and cholesterol content of 14 lipoprotein fractions weas measured using nuclear magnetic resonance (NMR) spectroscopy. Time since last meal was recorded by the examiner immediately before blood sampling.RESULTS: Following normal meals in age and sex-adjusted analyses and when compared with fasting levels, plasma triglycerides were higher for up to 5-6 h, and triglyceride content was higher for up to 6-7 h in HDL fractions, for up to 6-7 h in LDL + IDL fractions, and for up to 5-6 h in very-low-density lipoprotein (VLDL) fractions. Further, plasma cholesterol was lower for up to 2-3 h, and cholesterol content was lower for up to 0-1 h in HDL fractions and for up to 4-5 h in LDL + IDL fractions, while cholesterol content was higher for up to 4-5 h in VLDL fractions.CONCLUSIONS: Following normal meals, triglyceride content increases while cholesterol content decreases in HDL and LDL + IDL fractions.

KW - Humans

KW - Triglycerides

KW - Cross-Sectional Studies

KW - Cholesterol

KW - Lipoproteins, LDL

KW - Lipoproteins, VLDL

KW - Cholesterol, HDL

U2 - 10.1016/j.atherosclerosis.2023.117316

DO - 10.1016/j.atherosclerosis.2023.117316

M3 - Article (Academic Journal)

C2 - 37820443

SN - 0021-9150

VL - 383

JO - Atherosclerosis

JF - Atherosclerosis

M1 - 117316

ER -

Triglyceride content increases while cholesterol content decreases in HDL and LDL+IDL fractions following normal meals: The Copenhagen General Population Study of 25,656 individuals

Abstract

Bibliographical note

Research Groups and Themes

Keywords

Access to Document

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Projects

IEU: MRC Integrative Epidemiology Unit Quinquennial renewal

Cite this