Tunable genetic devices through simultaneous control of transcription and translation

Vittorio Bartoli, Grace Meaker, Mario Di Bernardo, Thomas E Gorochowski*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

63 Downloads (Pure)

Abstract

Synthetic genetic circuits allow us to modify the behavior of living cells. However, changes in environmental conditions and unforeseen interactions with the host cell can cause deviations from a desired function, resulting in the need for time-consuming reassembly to fix these issues. Here, we use a regulatory motif that controls transcription and translation to create genetic devices whose response functions can be dynamically tuned. This allows us, after construction, to shift the on and off states of a sensor by 4.5- and 28-fold, respectively, and modify genetic NOT and NOR logic gates to allow their transitions between states to be varied over a >6-fold range. In all cases, tuning leads to trade-offs in the fold-change and the ability to distinguish cellular states. This work lays the foundation for adaptive genetic circuits that can be tuned after their physical assembly to maintain functionality across diverse environments and design contexts.
Original languageEnglish
Article number2095 (2020)
Number of pages11
JournalNature Communications
Volume11
DOIs
Publication statusPublished - 29 Apr 2020

Structured keywords

  • BrisSynBio
  • Bristol BioDesign Institute

Fingerprint Dive into the research topics of 'Tunable genetic devices through simultaneous control of transcription and translation'. Together they form a unique fingerprint.

Cite this