Twist reverses muscle cell differentiation through transcriptional down-regulation of myogenin

Nikolaos P Mastroyiannopoulos, Antonis A Antoniou, Andrie Koutsoulidou, James B Uney, Leonidas A Phylactou

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Some higher vertebrates can display unique muscle regenerative abilities through dedifferentiation. Research evidence suggests that induced dedifferentiation can be achieved in mammalian cells. TWIST is a bHLH (basic helix-loop-helix) transcription factor that is expressed during embryonic development and plays critical roles in diverse developmental systems including myogenesis. Several experiments demonstrated its role in inhibition of muscle cell differentiation. We have previously shown that overexpression of TWIST can reverse muscle cell differentiation in the presence of growth factors. Here we show that TWIST reverses muscle cell differentiation through binding and down-regulation of myogenin. Moreover, it can reverse cellular morphology in the absence of growth factors.

Original languageEnglish
JournalBioscience Reports
Volume33
Issue number6
DOIs
Publication statusPublished - 3 Dec 2013

Keywords

  • Animals
  • Base Sequence
  • Binding Sites
  • Cell Differentiation
  • Cell Line
  • Cell Shape
  • Culture Media, Serum-Free
  • Down-Regulation
  • Gene Silencing
  • Mice
  • Molecular Sequence Data
  • Muscle Development
  • Myoblasts
  • Myogenin
  • Nuclear Proteins
  • Promoter Regions, Genetic
  • Protein Binding
  • Transcription, Genetic
  • Twist Transcription Factor

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