TY - JOUR
T1 - Type 1 Diabetes High-Risk HLA Class II Determination by Polymerase Chain Reaction Sequence-Specific Primers
AU - Aitken, Rachel J
AU - Mortimer, Georgina L
AU - Gillespie, Kathleen M
PY - 2015/12/13
Y1 - 2015/12/13
N2 - The only strategy to select individuals at increased risk for type 1 diabetes for primary prevention trials is through genetic risk assessment. While genome-wide association studies have identified more than 40 loci associated with type 1 diabetes, the single most important genetic determinants lie within the human leucocyte antigen gene family on chromosome 6.In this chapter we describe a protocol for a straightforward, cheap strategy to determine HLA class II mediated risk of type 1 diabetes. This method has proved robust for genotyping whole-genome-amplified DNA as well as DNA extracted directly from human tissues.
AB - The only strategy to select individuals at increased risk for type 1 diabetes for primary prevention trials is through genetic risk assessment. While genome-wide association studies have identified more than 40 loci associated with type 1 diabetes, the single most important genetic determinants lie within the human leucocyte antigen gene family on chromosome 6.In this chapter we describe a protocol for a straightforward, cheap strategy to determine HLA class II mediated risk of type 1 diabetes. This method has proved robust for genotyping whole-genome-amplified DNA as well as DNA extracted directly from human tissues.
U2 - 10.1007/7651_2015_307
DO - 10.1007/7651_2015_307
M3 - Article (Academic Journal)
C2 - 26659794
SN - 1064-3745
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -
