Projects per year
composition of DOM of both natural and anthropogenic origin derived from diffuse and point sources.
METHODS: DOM was extracted from riverine and treated sewage effluent using solid phase extraction (SPE) and analysed using dissolved organic carbon (DOC) analysis, direct infusion-high resolution Orbitrap™ mass spectrometry (DI-HRMS) and high-performance liquid chromatography (HPLC/HRMS). The data obtained were analysed using univariate and multivariate statistics to demonstrate differences in background DOM, anthropogenic inputs and in-river mixing. Compound identifications were achieved based on MS2 spectra searched against on-line databases.
RESULTS: DI-HRMS spectra showed the highly complex nature of all DOM SPE extracts. Classification and visualisation of extracts containing many thousands of individual compounds were achieved using PCA and hierarchical cluster analysis. Kruskal-Wallis analyses highlighted significant discriminating ions originating from the sewage treatment works for more in-depth investigation by HPLC/HRMS. The generation of MS2 spectra in HPLC/HRMS provided the basis for identification of anthropogenic compounds including; pharmaceuticals, illicit drugs, metabolites and polymers, although many thousands of compounds remain unidentified.
CONCLUSIONS: This new approach enables comprehensive analysis of DOM in extracts without any preconceived ideas of the compounds which may be present. This approach has the potential to be used as a high throughput, qualitative, screening method to determine if the composition of point sources differs from that of the receiving water bodies, providing a new approach to the identification of hitherto unrecognised organic contribution to water bodies.
- Dissolved organic matter
- sewage effluent
- data visualisation
- difference algorithm
DOMAINE: Characterisation of the nature, origins and ecological significance of dissolved organic matter in freshwater ecosystems
Johnes , P. J., Evershed, R. P., Jones, D., Maberly, S., Jickells, T., Yates, C. A., Lloyd, C. E. M., Glanville, H., Mackay, E., Harrison, R. V., Bayliss, C. E., Pemberton, J., Reay, M. K. M., Brailsford, F., McIntyre, C. A., Owen, A. T., Hopes, M., Evans, C., Fenner, N., Golyshin, P., Ferrer, M., Marshall, M. & Cooper, D.
1/04/14 → 31/12/19