Use of Mouse Carotid Artery Ligation Model of Intimal Thickening to Probe Vascular Smooth Muscle Cell Remodeling and Function in Atherosclerosis

Helen Williams; Bethan A Brown; Jason L Johnson; Sarah J George

doi:10.1007/978-1-0716-1924-7_33

Use of Mouse Carotid Artery Ligation Model of Intimal Thickening to Probe Vascular Smooth Muscle Cell Remodeling and Function in Atherosclerosis

Helen Williams, Bethan A Brown, Jason L Johnson, Sarah J George

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

1 Citation (Scopus)

Abstract

The thickening of the intima is a critical underlying component of atherosclerosis. Consequently, robust and reproducible animal models of intimal thickening are essential for a greater understanding of the mechanisms underlying the process of intimal thickening and to evaluate new approaches for the reduction of intimal thickening and thereby atherosclerosis. The ligation of the carotid artery in the mouse causes the thickening of the intimal layer of the artery. This model is relatively simple and is reproducible and therefore is a preferred and well-established model of intimal thickening. Here, we detail a protocol for carotid artery ligation in the mouse and methods for histological examination and quantification of intimal thickening.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	537-560
Number of pages	24
Volume	2419
DOIs	https://doi.org/10.1007/978-1-0716-1924-7_33
Publication status	Published - 1 Apr 2022

Publication series

Name	Methods in Molecular Biology
Volume	2419
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Funding Information:
Helen Williams is funded by British Heart Foundation project grant, PG/17/66/33216. Bethan Brown was funded by British Heart Foundation PhD studentship, FS/13/68/30489. Jason Johnson is funded by British Heart Foundation Senior Research Fellowship, FS/18/1/33234.

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Animals
Atherosclerosis/pathology
Carotid Arteries/pathology
Disease Models, Animal
Mice
Muscle, Smooth, Vascular/physiology
Myocytes, Smooth Muscle/pathology

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10.1007/978-1-0716-1924-7_33

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title = "Use of Mouse Carotid Artery Ligation Model of Intimal Thickening to Probe Vascular Smooth Muscle Cell Remodeling and Function in Atherosclerosis",

abstract = "The thickening of the intima is a critical underlying component of atherosclerosis. Consequently, robust and reproducible animal models of intimal thickening are essential for a greater understanding of the mechanisms underlying the process of intimal thickening and to evaluate new approaches for the reduction of intimal thickening and thereby atherosclerosis. The ligation of the carotid artery in the mouse causes the thickening of the intimal layer of the artery. This model is relatively simple and is reproducible and therefore is a preferred and well-established model of intimal thickening. Here, we detail a protocol for carotid artery ligation in the mouse and methods for histological examination and quantification of intimal thickening.",

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author = "Helen Williams and Brown, {Bethan A} and Johnson, {Jason L} and George, {Sarah J}",

note = "Funding Information: Helen Williams is funded by British Heart Foundation project grant, PG/17/66/33216. Bethan Brown was funded by British Heart Foundation PhD studentship, FS/13/68/30489. Jason Johnson is funded by British Heart Foundation Senior Research Fellowship, FS/18/1/33234. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

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doi = "10.1007/978-1-0716-1924-7_33",

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Use of Mouse Carotid Artery Ligation Model of Intimal Thickening to Probe Vascular Smooth Muscle Cell Remodeling and Function in Atherosclerosis. / Williams, Helen; Brown, Bethan A; Johnson, Jason L et al.
Methods in Molecular Biology. Vol. 2419 2022. p. 537-560 (Methods in Molecular Biology; Vol. 2419).

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

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KW - Disease Models, Animal

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KW - Myocytes, Smooth Muscle/pathology

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ER -

Use of Mouse Carotid Artery Ligation Model of Intimal Thickening to Probe Vascular Smooth Muscle Cell Remodeling and Function in Atherosclerosis

Abstract

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Bibliographical note

Keywords

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