Use of Mouse Carotid Artery Ligation Model of Intimal Thickening to Probe Vascular Smooth Muscle Cell Remodeling and Function in Atherosclerosis

Helen Williams; Bethan A Brown; Jason L Johnson; Sarah J George

doi:10.1007/978-1-0716-1924-7_33

Use of Mouse Carotid Artery Ligation Model of Intimal Thickening to Probe Vascular Smooth Muscle Cell Remodeling and Function in Atherosclerosis

Helen Williams, Bethan A Brown, Jason L Johnson, Sarah J George^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

1 Citation (Scopus)

Abstract

The thickening of the intima is a critical underlying component of atherosclerosis. Consequently, robust and reproducible animal models of intimal thickening are essential for a greater understanding of the mechanisms underlying the process of intimal thickening and to evaluate new approaches for the reduction of intimal thickening and thereby atherosclerosis. The ligation of the carotid artery in the mouse causes the thickening of the intimal layer of the artery. This model is relatively simple and is reproducible and therefore is a preferred and well-established model of intimal thickening. Here, we detail a protocol for carotid artery ligation in the mouse and methods for histological examination and quantification of intimal thickening.

Original language	English
Title of host publication	Atherosclerosis
Subtitle of host publication	Methods and Protocols
Editors	Dipak Ramji
Publisher	Humana Press
Pages	537-560
Number of pages	24
ISBN (Electronic)	9781071619247
ISBN (Print)	9781071619230
DOIs	https://doi.org/10.1007/978-1-0716-1924-7_33
Publication status	Published - 4 Mar 2022

Publication series

Name	Methods in Molecular Biology
Volume	2419
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Animals
Atherosclerosis/pathology
Carotid Arteries/pathology
Disease Models, Animal
Mice
Muscle, Smooth, Vascular/physiology
Myocytes, Smooth Muscle/pathology

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Williams, H., Brown, B. A., Johnson, J. L., & George, S. J. (2022). Use of Mouse Carotid Artery Ligation Model of Intimal Thickening to Probe Vascular Smooth Muscle Cell Remodeling and Function in Atherosclerosis. In D. Ramji (Ed.), Atherosclerosis: Methods and Protocols (pp. 537-560). (Methods in Molecular Biology; Vol. 2419). Humana Press. https://doi.org/10.1007/978-1-0716-1924-7_33

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abstract = "The thickening of the intima is a critical underlying component of atherosclerosis. Consequently, robust and reproducible animal models of intimal thickening are essential for a greater understanding of the mechanisms underlying the process of intimal thickening and to evaluate new approaches for the reduction of intimal thickening and thereby atherosclerosis. The ligation of the carotid artery in the mouse causes the thickening of the intimal layer of the artery. This model is relatively simple and is reproducible and therefore is a preferred and well-established model of intimal thickening. Here, we detail a protocol for carotid artery ligation in the mouse and methods for histological examination and quantification of intimal thickening.",

keywords = "Animals, Atherosclerosis/pathology, Carotid Arteries/pathology, Disease Models, Animal, Mice, Muscle, Smooth, Vascular/physiology, Myocytes, Smooth Muscle/pathology",

author = "Helen Williams and Brown, \{Bethan A\} and Johnson, \{Jason L\} and George, \{Sarah J\}",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

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language = "English",

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Williams, H, Brown, BA, Johnson, JL & George, SJ 2022, Use of Mouse Carotid Artery Ligation Model of Intimal Thickening to Probe Vascular Smooth Muscle Cell Remodeling and Function in Atherosclerosis. in D Ramji (ed.), Atherosclerosis: Methods and Protocols. Methods in Molecular Biology, vol. 2419, Humana Press, pp. 537-560. https://doi.org/10.1007/978-1-0716-1924-7_33

Use of Mouse Carotid Artery Ligation Model of Intimal Thickening to Probe Vascular Smooth Muscle Cell Remodeling and Function in Atherosclerosis. / Williams, Helen; Brown, Bethan A; Johnson, Jason L et al.
Atherosclerosis: Methods and Protocols. ed. / Dipak Ramji. Humana Press, 2022. p. 537-560 (Methods in Molecular Biology; Vol. 2419).

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

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AU - Johnson, Jason L

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PY - 2022/3/4

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N2 - The thickening of the intima is a critical underlying component of atherosclerosis. Consequently, robust and reproducible animal models of intimal thickening are essential for a greater understanding of the mechanisms underlying the process of intimal thickening and to evaluate new approaches for the reduction of intimal thickening and thereby atherosclerosis. The ligation of the carotid artery in the mouse causes the thickening of the intimal layer of the artery. This model is relatively simple and is reproducible and therefore is a preferred and well-established model of intimal thickening. Here, we detail a protocol for carotid artery ligation in the mouse and methods for histological examination and quantification of intimal thickening.

AB - The thickening of the intima is a critical underlying component of atherosclerosis. Consequently, robust and reproducible animal models of intimal thickening are essential for a greater understanding of the mechanisms underlying the process of intimal thickening and to evaluate new approaches for the reduction of intimal thickening and thereby atherosclerosis. The ligation of the carotid artery in the mouse causes the thickening of the intimal layer of the artery. This model is relatively simple and is reproducible and therefore is a preferred and well-established model of intimal thickening. Here, we detail a protocol for carotid artery ligation in the mouse and methods for histological examination and quantification of intimal thickening.

KW - Animals

KW - Atherosclerosis/pathology

KW - Carotid Arteries/pathology

KW - Disease Models, Animal

KW - Mice

KW - Muscle, Smooth, Vascular/physiology

KW - Myocytes, Smooth Muscle/pathology

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DO - 10.1007/978-1-0716-1924-7_33

M3 - Chapter in a book

C2 - 35237987

SN - 9781071619230

T3 - Methods in Molecular Biology

SP - 537

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BT - Atherosclerosis

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PB - Humana Press

ER -

Use of Mouse Carotid Artery Ligation Model of Intimal Thickening to Probe Vascular Smooth Muscle Cell Remodeling and Function in Atherosclerosis

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