Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells

Imogen Sparkes*, Rhiannon R White, Benjamin Coles, Stanley W Botchway, Andy Ward

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

4 Citations (Scopus)
247 Downloads (Pure)

Abstract

Optical tweezers have been used to trap and micromanipulate several biological specimens ranging from DNA, macromolecules, organelles to single celled organisms. Using a combination of the refraction and scattering of laser light from a focused laser beam, refractile objects are physically captured and can be moved within the surrounding media. The technique is routinely used to determine biophysical properties such as the forces exerted by motor proteins. Here, we describe how optical tweezers combined with total internal reflection fluorescence (TIRF) microscopy can be used to assess physical interactions between organelles, more specifically the ER and Golgi bodies in plant cells.
Original languageEnglish
Title of host publicationThe Plant Endoplasmic Reticulum
Subtitle of host publicationMethods and Protocols
EditorsChris Hawes, Verena Kriechbaumer
PublisherHumana Press
Pages167-178
Number of pages12
Volume1691
ISBN (Electronic)9781493973897
ISBN (Print)9781493973880
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1691
ISSN (Print)1064-3745

Keywords

  • Optical trap
  • ER
  • Golgi
  • Tweezers
  • GFP

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