Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells

Imogen Sparkes; Rhiannon R White; Benjamin Coles; Stanley W Botchway; Andy Ward

doi:10.1007/978-1-4939-7389-7_13

Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells

Imogen Sparkes^*, Rhiannon R White, Benjamin Coles, Stanley W Botchway, Andy Ward

^*Corresponding author for this work

School of Biological Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

6 Citations (Scopus)

370 Downloads (Pure)

Abstract

Optical tweezers have been used to trap and micromanipulate several biological specimens ranging from DNA, macromolecules, organelles to single celled organisms. Using a combination of the refraction and scattering of laser light from a focused laser beam, refractile objects are physically captured and can be moved within the surrounding media. The technique is routinely used to determine biophysical properties such as the forces exerted by motor proteins. Here, we describe how optical tweezers combined with total internal reflection fluorescence (TIRF) microscopy can be used to assess physical interactions between organelles, more specifically the ER and Golgi bodies in plant cells.

Original language	English
Title of host publication	The Plant Endoplasmic Reticulum
Subtitle of host publication	Methods and Protocols
Editors	Chris Hawes, Verena Kriechbaumer
Publisher	Humana Press
Pages	167-178
Number of pages	12
Volume	1691
ISBN (Electronic)	9781493973897
ISBN (Print)	9781493973880
DOIs	https://doi.org/10.1007/978-1-4939-7389-7_13
Publication status	Published - 2018

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
Volume	1691
ISSN (Print)	1064-3745

Keywords

Optical trap
ER
Golgi
Tweezers
GFP

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10.1007/978-1-4939-7389-7_13

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Sparkes, I., White, R. R., Coles, B., Botchway, S. W., & Ward, A. (2018). Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells. In C. Hawes, & V. Kriechbaumer (Eds.), The Plant Endoplasmic Reticulum: Methods and Protocols (Vol. 1691, pp. 167-178). (Methods in Molecular Biology; Vol. 1691). Humana Press. https://doi.org/10.1007/978-1-4939-7389-7_13

Sparkes, Imogen ; White, Rhiannon R ; Coles, Benjamin et al. / Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells. The Plant Endoplasmic Reticulum: Methods and Protocols. editor / Chris Hawes ; Verena Kriechbaumer. Vol. 1691 Humana Press, 2018. pp. 167-178 (Methods in Molecular Biology).

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abstract = "Optical tweezers have been used to trap and micromanipulate several biological specimens ranging from DNA, macromolecules, organelles to single celled organisms. Using a combination of the refraction and scattering of laser light from a focused laser beam, refractile objects are physically captured and can be moved within the surrounding media. The technique is routinely used to determine biophysical properties such as the forces exerted by motor proteins. Here, we describe how optical tweezers combined with total internal reflection fluorescence (TIRF) microscopy can be used to assess physical interactions between organelles, more specifically the ER and Golgi bodies in plant cells.",

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Sparkes, I, White, RR, Coles, B, Botchway, SW & Ward, A 2018, Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells. in C Hawes & V Kriechbaumer (eds), The Plant Endoplasmic Reticulum: Methods and Protocols. vol. 1691, Methods in Molecular Biology, vol. 1691, Humana Press, pp. 167-178. https://doi.org/10.1007/978-1-4939-7389-7_13

Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells. / Sparkes, Imogen; White, Rhiannon R; Coles, Benjamin et al.

The Plant Endoplasmic Reticulum: Methods and Protocols. ed. / Chris Hawes; Verena Kriechbaumer. Vol. 1691 Humana Press, 2018. p. 167-178 (Methods in Molecular Biology; Vol. 1691).

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

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AU - Ward, Andy

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AB - Optical tweezers have been used to trap and micromanipulate several biological specimens ranging from DNA, macromolecules, organelles to single celled organisms. Using a combination of the refraction and scattering of laser light from a focused laser beam, refractile objects are physically captured and can be moved within the surrounding media. The technique is routinely used to determine biophysical properties such as the forces exerted by motor proteins. Here, we describe how optical tweezers combined with total internal reflection fluorescence (TIRF) microscopy can be used to assess physical interactions between organelles, more specifically the ER and Golgi bodies in plant cells.

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KW - ER

KW - Golgi

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Sparkes I, White RR, Coles B, Botchway SW, Ward A. Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells. In Hawes C, Kriechbaumer V, editors, The Plant Endoplasmic Reticulum: Methods and Protocols. Vol. 1691. Humana Press. 2018. p. 167-178. (Methods in Molecular Biology). Epub 2017 Oct 17. doi: 10.1007/978-1-4939-7389-7_13

Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells

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